Enables highly sensitive detection of cancer markers in urine samples
Air-dried | Ultraplex ™ (QuantBio)
Three tumor-related mRNA markers (CDC2 kinase, IGFBP5 and MDK) were amplified from total RNA (from a bladder cancer patient) in a triplex reaction using air-dried Air-Dryable ™ Direct RNA/DNA qPCR Urine ( red ) or Ultraplex ™ (QuantBio, grey ) in 10% human urine. The results illustrate that Air-Dryable ™ Direct RNA/DNA qPCR Urine is able to detect cancer related RNA markers from urine faster and with high sensitivity compared to the other mix.
Fast time to results using very fast cycling conditions A) Air-Dryable ™ Direct DNA qPCR Urine (MDX150)
B) Air-Dryable ™ Direct RNA/DNA qPCR Urine (MDX151)
A) Targets from Neisseria gonorrhoeae, Chlamydia trachomatis and group B streptococcus (GBS) were amplified in a triplex reaction in the presence of 25% human urine under standard cycling conditions: 40 cycles of 10 sec @ 95°C and 30 sec @ 60°C and fast cycling conditions: 3 min at 95°C followed by 40 cycles of 1 sec @ 95°C and 1 sec @ 60 °C. B) Three mammalian RNA targets were amplified in a triplex reaction in the presence of 5% human urine under standard cycling conditions: 10 min reverse transcription step followed by 40 cycles of 10 sec @ 95°C and 30 sec @ 60°C and fast cycling conditions: 5 min reverse transcription step followed by 40 cycles of 1 sec @ 95°C and 1 sec @ 60°C. All reactions were carried out in the Quantstudio ™ 7 Flex Real-Time PCR System. The results illustrate the ability of Air-Dryable Direct Urine mixes to deliver the fastest possible results, without compromising the sensitivity and reproducibility of an assay.
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