Food &Water Safety Reagents for Assay Development
Meridian Life Science, Inc.
ISO 9001 Certified & cGMP Compliant
Food &Water Safety Reagents for Assay Development
Innovative Solutions. Trusted Partner. ® Life Science, Inc. ®
www.MeridianLifeScience.com
Life Science, Inc. ®
Meridian Life Science, Inc. (MLS) is a leading large scale manufacturer of antibodies, viral antigens, recombinant proteins, and critical assay reagents. We provide contract R&D, process development, and manufacturing services to the biopharmaceutical and in vitro diagnostic markets along with cGMP biologics manufacturing for Phase I/II clinical trials. MLS has been providing innovative life science solutions and building trusted partnerships for almost thirty years. Our focus is to offer products and services that help to advance the development of diagnostic assays and vaccine development. • Commercial scale manufacturing of antigens and antibodies with protein purification expertise • Full line of immunoassay reagents including blockers, antigens and antibodies
• Large scale production of reagents for molecular assays
• Technical support with assay development expertise
• Dedicated R&D and ManufacturingTeams
ISO 9001 Certified & cGMP Compliant
• Robust and Mature Quality System
IMMUNODIAGNOSTICS Antigens & Antibodies Recombinant Proteins Blocking Agents
CONTRACT SERVICES Antigens & Antibodies cGMP Phase I/II Viral Vaccines Cell & Viral Banking
MOLECULAR BIOLOGY Nucleotides Enzymes PCR / qPCR Reagents
CORE EXPERTISE Cell Culture | Protein Purification | In Vitro & In Vivo MAb Mfg R&D Contract Mfg | Nucleotide Chemistry | PCR / qPCR Enzymes
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Capabilities
MANUFACTURING SITES
LONDON, UK PCR / qPCR Assay Development RNA Analysis DNA MW Marker Mfg
MEMPHIS,TN Viral Antigens
Recombinant Proteins In Vitro Antibody Mfg HAMA Blocking Agents Antibody Purification Contract R&D and PD Cell and Viral Banking cGMP Vaccine Mfg
BERLIN, DE Large Scale Nucleotides Mfg PCR Enzyme Mfg Protein Expression and Purification DNA MW Markers
BOCA RATON, FL Ascites Production Large Scale MAb Mfg 55,000 Mice (BALB/c, CAF1)
SYDNEY, AUS Competent Cells Mfg Diagnostic Extraction Controls
Parent Company: Meridian Bioscience, Inc. Diagnostic Test Kits | Founded in 1977; IPO in 1986 | Nasdaq: VIVO Headquartered in Cincinnati, OH | Employees: 500+ | International Presence, 60+ countries
OVERVIEW OF CAPABILITIES
PRODUCTION SYSTEMS Tissue culture flasks Spinner flasks Roller bottles (smooth and ribbed) Cell factories WAVE Bioreactors TM Fermentors
EXPRESSION SYSTEMS Mammalian (CHO, Vero, NS0, etc.) Bacterial ( E.coli ) Yeast ( P. pastoris, S. cerevisiae, etc.) Baculovirus in Sf9 Egg-based Virus Production Vaccinia & Adeno
PURIFICATION METHODS Ultrafiltration Diafiltration TFF (plate or hollow fiber)
Centrifugation/ultracentrifugation ÄKTA FPLC systems for SEC, Affinity, Ion exchange, HIC Dialysis, ultracentrifugation, tangential flow Expanded bed chromatography Centrifugation, gradient centrifugation Microfluidization, freeze/thaw, chemical lysis
QUALITY CONTROL AND ANALYTICAL TESTING Mature quality system with dedicated QA/QC and Regulatory team In-house testing (e.g. protein concentration, HPLC, ELISA, SDS-PAGE, Western blot) ISO 9001 Quality System Regulations - Applicable sections of 21 CFR 820 Current Good Manufacturing Practices - Applicable Sections of 21 CFR 210, 211
WAVE is a trademark of GE Healthcare
www.MeridianLifeScience.com
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Capabilities
REAGENTS FOR FOOD & WATER SAFETY TESTING
In the United States, foodborne disease causes an estimated 48 million illnesses and 3,000 deaths annually, with the economic costs estimated at $152 billion to $1.4 trillion every year. Food safety is a major global, public health concern and requires a highly integrated network of initiatives and practices to assure food safety throughout the entire supply chain from the farm to the consumer. Adding to the complication is the continued increase in international trade and challenges faced governing the import/export regulations of food and agricultural products shipped between countries. Sources of drinking water are also subject to contamination and require appropriate treatment to remove disease-causing contaminants. There are many sources of water contamination including naturally occurring chemicals and minerals (for example, arsenic, radon, uranium), local land use practices (fertilizers, pesticides, concentrated feeding operations), manufacturing processes, and sewer overflows or wastewater releases. The presence of contaminants in water can lead to adverse health effects, including gastrointestinal illness, reproductive problems, and neurological disorders. The detection of microorganisms in food and water is an essential part of any quality control or food safety plan. Traditional methods of detecting pathogenic bacteria are often impractical and time-consuming because of the need for growth in culture media followed by isolation, biochemical and/or serological identification, and in some cases, sub- specific characterization. Advances in technology such as antibody and DNA-based assays have made detection and identification faster, more sensitive, more specific, and more convenient than traditional assays. However, even with the application of modern laboratory techniques, there still remains a considerable challenge in identifying the microorganisms responsible for outbreaks. This suggests a large unmet need for better diagnostic tools for foodborne and waterborne diseases. Meridian Life Science (MLS) is a leading large scale manufacturer of antibodies, viral antigens, recombinant proteins, and critical assay reagents. For almost thirty years, MLS has provided innovative life science solutions for in vitro diagnostic, biopharmaceutical, and biotech manufacturers around the world. The company’s reagents are integral components of commercially available human and animal in vitro diagnostic kits, in-process testing protocols, and high-throughput screening applications worldwide. MLS’ products are designed to help maximize the accuracy, consistency and cost-effectiveness of food and water safety testing.
FOOD & WATER SAFETY CATEGORIES Antibiotics Gram Negative Bacteria Gram Positive Bacteria Viruses Protozoans (Waterborne) Natural Toxins
Top Five Foodborne pathogens causing the most illnesses, hospitalizations, and deaths each year
Estimated # of illnesses
90% Credible Interval 3,227,078 – 8,309,480
Pathogen
%
Norovirus
5,461,731
58
Salmonella, nontyphoidal
644,786 – 1,679,667
1,027,561
11
Clostridium perfringens
192,316 – 2,483,309
965,958
10
337,031 – 1,611,083
Campylobacter spp. 845,024
9
Staphylococcus aureus
72,341 – 529,417
241,148
3
Subtotal
91
Thirty-one pathogens are known to cause foodborne illness. Eight of those pathogens account for the vast majority of illnesses, hospitalizations, and deaths. The table above represents the top five pathogens contributing to acquired foodborne illnesses in the United States (Source: CDC).
For a complete list please visit us at www.MeridianLifeScience.com
Other Product Categories available • Infectious Disease
• Cancer & Tumor Markers
• Cardiac & Cholesterol • Blocking Agents • Clinical Chemistry
• Hormones
• Drugs of Abuse
Centers for Disease Control and Prevention (2012). Estimates of Foodborne Illness in the United States. Retrieved from http://www.cdc.gov/foodborneburden/ López-Campos, G., Martínez -Suárez, J.V., Aguado-Urda M., and López-Alonso, V. (2012). Microarray Detection and Characterization of Bacterial Foodborne Pathogens. Springer: New York. Riemann , H.P. Cliver, D.O. (2005). Foodborne Infections and Intoxications. 3d ed. Academic Press (Elsevier): London, Amsterdam.
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antibiotics
products
antibiotics Antibiotics are a commonly employed group of drugs in farm animals used to stimulate their growth and reduce infection and stock loss. However, the use of antibiotics in food production can pose certain public health risks, as it unintentionally selects for antibiotic resistant bacteria leading to their contamination in animal-derived food. This can create a health hazard if humans become infected with a microorganism strain that cannot be controlled by available treatments. Amidst the threat of increased microbial antibiotic resistance, the FDA issued new guidelines in 2011 urging meat producers to refrain from using antibiotics to promote livestock growth, calling the practice an “urgent public health issue” as a result of their potential to promote the emergence of multi-drug resistant pathogens. Antibiotic residues can be detected in a range of tissues using various analytical methods such as microbial and enzyme inhibition tests, receptor binding assays, immunoassays, biosensors and HPLC. Sensitive and rapid assays that can be used on the farm, truck and in the plant are needed in order to minimize the impact of potentially transmitting antibiotic-resistant bacteria to humans. Ellis, R.L. American Analytical Detection Systems: Present and Future. Academy of Veterinary Pharmacology & Therapeutics Symposium. Blacksburg, Virginia. 1998 Food and Drug Administration (2012). The Judicious Use of Medically Important Antimicrobial Drugs in Food-Producing Animals (#209). Retrieved from http://www.fda.gov/downloads/animalveterinary/guidancecomplianceenforcement/guidanceforindustry/ucm216936.pdf Natural News. http://www.naturalnews.com/030132_antibiotics_meat.html#ixzz1znNPBHMZ (July 2012)
Ceftiofur ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
G13034M Ceftiofur (includes BSA conjugate)
Mouse CH-2034
EIA
N Dil.Ascites
NaN3
Penicillin ANTIBODIES Catalog # Specificity G45216M Penicillin G45060M Penicillin
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Mouse 112-16229
N/A EIA
N Purified N Purified
NaN3 NaN3
Mouse
Pen9
Penicillin, Ampicillin, Amoxicillin, Cloxacillin, Hetacillin (includes BSA conjugate)
G13011M
Mouse CH-2011
EIA
N Dil.Ascites
NaN3
G01246M Penicillin (Thiazolidine Ring)
Mouse B612M EIA
N Purified
NaN3
www.MeridianLifeScience.com
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Gram Negative Bacteria
products
Gram Negative Bacteria Gram-negative bacterial pathogens are a worldwide cause of morbidity and mortality. They are responsible for a wide variety of infections including foodborne and waterborne illness, respiratory infections, and sexually transmitted diseases. Environmental sources of the organisms include raw meat or other raw food items, juice and water. The pathogenic capability of Gram-negative bacteria is associated with a unique component of its cell envelope, in particular the lipopolysaccharide layer (also known as LPS or endotoxin) which produces host toxicity. Treating Gram-negative infections can be difficult due to this LPS layer which also serves to protect the bacteria through enabling its resistance to many drugs such as antibiotics, detergents and chemicals. The spread of antibiotic and drug resistance in these bacteria is complicating their treatment and forcing doctors to find alternative therapies or rely on outdated antibiotics that can cause kidney and nerve damage. Traditional detection methods for Gram- negative bacteria that cause foodborne and waterborne outbreaks rely on time-consuming stool cultures and the error-prone Gram stain. Alternative methods which are accurate and rapid are critical to effectively manage the treatment of outbreaks where the infectious dose can be as low as 1 –10 cells.
National Institute of Allergy and Infectious Disease (2012). Antimicrobial Drug Resistance. Retrieved from http://www.niaid.nih.gov/topics/antimicrobialresistance/examples/gramnegative/Pages/default.aspx Todar, K. Bacterial Resistance to Antibiotics. The Microbial World. (2009). July 2012. Retrieved from http://textbookofbacteriology.net/tuberculosis.html
Campylobacter jejuni ANTIBODIES Catalog # Specificity C01614M Campylobacter jejuni C65717M Campylobacter jejuni C65727M Campylobacter jejuni C86082M Campylobacter jejuni C86083M Campylobacter jejuni C86326M Campylobacter jejuni
Host / Source Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
57-24
EIA
N Dil.Sup.
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 None
Mouse BDI717 Mouse BDI727
EIA,WB N Purified EIA,WB N Purified
Mouse B082M EIA,WB N Purified Mouse B083M EIA,WB N Purified Mouse B140M EIA,WB N Purified Mouse B617M EIA,IFA N Purified
C01482M Campylobacter jejuni Types 1 & 2, non-flagellar
ANTIGENS Catalog # Specificity
Host / Source Culture
Dry Ice
Format
Preservative
R14900
Campylobacter jejuni Antigen
Purified
Y
None
Gram Negative Endotoxin ANTIBODIES Catalog # Specificity
Host / Source Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
C55156M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55158M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55159M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55160M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55304M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55305M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55308M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55509M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C01575M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55157M Gram Negative Endotoxin (E. coli LPS)
301 303 306 507 304 305 308 509 305 302
EIA EIA EIA EIA EIA EIA EIA EIA EIA EIA
N Ascites N Ascites N Ascites N Ascites N Ascites N Ascites N Ascites N Ascites N Purified N Ascites
NaN3 NaN3 None None NaN3 None NaN3 None None None
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Gram Negative Bacteria
products
E. coli ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C86154M E. coli heat-labile Enterotoxin A-chain C86157M E. coli heat-labile Enterotoxin A-chain C86232M E. coli heat-labile Enterotoxin A-chain C86222M E. coli heat-labile Enterotoxin B-chain C86271M E. coli heat-labile Enterotoxin B-chain C86272M E. coli heat-labile Enterotoxin B-chain
Mouse B154M EIA Mouse B157M WB Mouse B123M EIA
N Purified N Purified N Purified
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3
Mouse B222M EIA,WB N Purified Mouse B271M EIA,WB N Purified Mouse B221M EIA,WB N Purified
C55184M E. coli K99 pili C55840M E. coli K99 pili C01533M E. coli O157 C01534M E. coli O157 C01535M E. coli O157 C01536M E. coli O157 C01537M E. coli O157 C65160M E. coli O157 C65310M E. coli O157 MAV119-499 E. coli O157:H7
Mouse Mouse
401 402
EIA EIA
N Purified N Purified N Purified N Purified N Purified N Purified N Purified
Mouse B835M EIA,IFA,Pr Mouse B836M EIA,IFA,Pr Mouse B837M EIA,IFA,Pr Mouse B838M EIA,IFA,Pr Mouse B839M EIA,IFA,Pr
Mouse BDI160
EIA,IFA N Purified
Mouse
3011
IFA EIA
N Purified N Purified
Mouse 300-13499
Matched antibody pairs Specificity
Capture Antibody
Detection Antibody
C01534M C01535M C01536M C01537M
C01533M C01533M C01533M C01534M
E. coli O157
Legionella pneumophila ANTIBODIES Catalog # Specificity C01590M Legionella pneumophila LPS C01591M Legionella pneumophila LPS
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Mouse B1252M EIA,IFA,Pr Mouse B1254M EIA,IFA,Pr
N Purified N Purified N Purified N Purified
NaN3 NaN3 NaN3 NaN3 NaN3
C86515M Legionella pneumophila LPS Philadelphia 1 strain C86135M Legionella pneumophila LPS Philadelphia 1 strain
Mouse Mouse
5F4
EIA,Pr DB,Pr
2F10
C01479M Legionella pneumophila serogroup 1
Mouse B597M EIA,IFA N Purified
ANTIGENS Catalog # Specificity
Host / Source
Dry Ice
Format
Preservative
R14610
Legionella pneumophila Serovars 1-7
Culture Whole Cell
Y
None
Matched antibody pairs
Specificity
Capture Antibody Detection Antibody
C86135M C01590M
C86515M C01591M
Legionella pneumophila LPS
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Gram Negative Bacteria
products
Lipopolysaccharide (LPS) Lipid A ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Lipopolysaccharide (LPS gram negative bacteria) (Lipid A)
C01613M
Mouse
26-5
EIA
N Purified
NaN3
Pseudomonas mallei (BURKHOLDERIA MALLEI) ANTIBODIES Catalog # Specificity Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C86315M Pseudomonas mallei
Mouse
3D11
EIA,WB N Purified
NaN3
Salmonella ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C01553M Salmonella Paratyphi A C65301M Salmonella species C65321M Salmonella species C87101M Salmonella species
Mouse B926M EIA,IFA N Purified
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3
Mouse
6301
EIA EIA EIA
N Purified N Purified N Supernatant
Mouse BID32 Mouse IFR0111
C86109M Salmonella species (A,B & D Groups) C65635M Salmonella species (A,B,C,D & E Groups) C65957M Salmonella species (A,B,C,D,E,F & G Groups) C65958M Salmonella species (A,B,C,D,E,F & G Groups) C01358M Salmonella species (A,B,C,D,E,F & G Groups) C01359M Salmonella species (A,B,C,D,E,F & G Groups) C01360M Salmonella species (A,B,C,D,E,F & G Groups) C01361M Salmonella species (A,B,C,D,E,F & G Groups)
Mouse 10D9H DB,EIA,IFA N Purified Mouse 5D12A EIA,IFA,WB N Purified Mouse B495M EIA,IFA N Purified Mouse B395M EIA,IFA N Purified
Mouse B343M EIA,Pr Mouse B345M EIA,IFA,Pr Mouse B346M EIA,IFA,Pr Mouse B347M EIA,IFA,Pr
N Purified N Purified N Purified N Purified
C01478M Salmonella typhi C01362M Salmonella typhi (LPS) C86309M Salmonella typhimurium-LPS
Mouse B596M EIA,IFA N Purified Mouse B348M EIA,IFA N Purified
Mouse
1E6
EIA
N Purified
ANTIGENS Catalog # Specificity
Host / Source
Dry Ice
Format
Preservative
R84500
Teichoic Acid, Staphylococcus aureus (Wood Strain)
St. aureus
Purified
N None
Matched antibody pairs
Specificity
Capture Antibody
Detection Antibody
C01358M C01358M C01359M C01359M
C01360M C01361M C01360M C01361M
Salmonella species
Shigella boydii ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C01480M Shigella boydii
Mouse B598M IFA
N Purified
NaN3
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Gram positive Bacteria
products
Gram Positive Bacteria Gram-positive organisms are the leading pathogens of humans, producing an estimated one-third of all bacterial infections. Treatment of Gram-positive infections with standard antibiotics has recently become problematic as many of these pathogens have developed antimicrobial resistance. In the food industry, Gram-positive bacteria are generally spread through cross contamination between people, however, antimicrobial-resistance in Gram-positive bacteria is reported with increasing frequency in strains isolated from food animals. This increase constitutes a potential risk that resistant strains or resistant genes might spread to humans via the food chain. Foods frequently involved in foodborne intoxications caused by Gram-positive bacteria include meat, poultry, eggs, fish, and dairy products. This rising occurrence of drug resistance in these bacteria combined with their prevalence of infection has created the need for quick and efficient methods for identifying the gram-positive bacteria down to the strain level.
Todar, K. Bacterial Resistance to Antibiotics. The Microbial World. (2009). July 2012. Retrieved from http://textbookofbacteriology.net/tuberculosis.html Todar, K. Important Groups of Procaryotes. Todar’s Online Textbook of Bacteriology. July 2012. Retrieved from http://textbookofbacteriology.net/tuberculosis.html
Bacillus anthracis (Anthrax) ANTIBODIES Catalog # Specificity C86542M Bacillus anthracis Lethal Factor (Anthrax) C86543M Bacillus anthracis Lethal Factor (Anthrax) C86701M Bacillus anthracis Lethal Factor (Anthrax) C86710M Bacillus anthracis Lethal Factor (Anthrax) C86110M Bacillus anthracis Protective Antigen (Anthrax) C86201M Bacillus anthracis Protective Antigen (Anthrax) C86301M Bacillus anthracis Protective Antigen (Anthrax) C86410M Bacillus anthracis Protective Antigen (Anthrax) C86501M Bacillus anthracis Protective Antigen (Anthrax) C86601M Bacillus anthracis Protective Antigen (Anthrax) C86613M Bacillus anthracis Protective Antigen (Anthrax) C86702M Bacillus anthracis Spore Antigen (Anthrax) C86910M Bacillus anthracis Spore Antigen (Anthrax)
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Mouse B743M EIA,Pr Mouse B414M EIA,Pr
N Purified N Purified
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3
Mouse BAL0105 EIA,Pr,WB N Purified Mouse BAL0106 EIA,Pr,WB N Purified
Mouse BAP0101
EIA,WB N Purified
Mouse BAP0102 EIA,Pr,WB N Purified Mouse BAP0103 EIA,Pr,WB N Purified
Mouse BAP0104
EIA,WB N Purified
Mouse BAP0105 EIA,Pr,WB N Purified Mouse BAP0106 EIA,Pr,WB N Purified
Mouse Mouse Mouse
C3
EIA,Pr,WB N Purified EIA,Pr,WB N Purified EIA,Pr,WB N Purified
SA27 SA26
Matched antibody pairs
Specificity
Capture Antibody
Detection Antibody
C86710M C86543M C86613M C86613M C86501M
C86701M C86542M C86201M C86301M C86601M
Bacillus anthracis Lethal Factor
Bacillus anthracis Protective Antigen
Bacillus anthracis Spore Antigen
C86910M
C86702M
www.MeridianLifeScience.com
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Gram Positive Bacteria
products
Clostridium ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C01370M Clostridium botulinum Toxin E (a.a. 2-17) C01371M Clostridium botulinum Toxin E (a.a. 2-17) C01372M Clostridium botulinum Toxin A (a.a. 1280-1292) C01373M Clostridium botulinum Toxin A (a.a. 1280-1292) C01374M Clostridium botulinum Toxin B (a.a. 1278-1291)
Mouse B369M EIA,IFA,RIA N Supernatant Mouse B367M EIA,IFA,RIA N Supernatant Mouse B365M EIA,IFA,RIA N Supernatant Mouse B364M EIA,IFA,RIA N Supernatant Mouse B362M EIA,IFA,RIA N Supernatant
None None None None None
Clostridium botulinum Type A Neurotoxin Heavy Chain (a.a. 1177-1195) Clostridium botulinum Type A Neurotoxin Heavy Chain (a.a. 869-887)
C86029M
Mouse B29M EIA,WB N Purified
NaN3
C86033M
Mouse B33M EIA,WB N Purified
NaN3
C86211M Clostridium botulinum Type A Toxoid C86468M Clostridium botulinum Type A Toxoid C86018M Clostridium botulinum Type B Toxoid C86336M Clostridium botulinum Type B Toxoid C86042M Clostridium botulinum Type E Toxoid C86169M Clostridium botulinum Type E Toxoid
Mouse KBA211 Mouse KBA468 Mouse KBB18 Mouse KBB36 Mouse KBE42 Mouse KBE169 Mouse BDI555 Mouse BDI517 Mouse PCG4.1 Mouse PCG4.1
EIA,Pr EIA,Pr EIA,Pr EIA,Pr EIA,Pr EIA,Pr
N Purified N Purified N Purified N Purified N Purified N Purified N Purified N Purified
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 None NaN3 NaN3 NaN3
C65555M Clostridium difficile Toxin A C65715M Clostridium difficile Toxin A C70472M Clostridium difficile Toxin A C70517M Clostridium difficile Toxin A C01483M Clostridium difficile Toxin A C65423M Clostridium difficile Toxin B C65424M Clostridium difficile Toxin B C65426M Clostridium difficile Toxin B C65427M Clostridium difficile Toxin B C65429M Clostridium difficile Toxin B C70885M Clostridium difficile Toxin B C70888M Clostridium difficile Toxin B
EIA EIA EIA EIA
Y Y
Ascites Purified
Mouse B618M IFA Mouse B423M EIA Mouse B424M EIA Mouse B426M EIA Mouse B427M EIA Mouse B428M EIA
N Purified N Purified N Purified N Purified N Purified N Purified
Mouse 5A8-E11 Mouse 5A8-E11
EIA EIA
Y Y
Ascites Purified
C01393M Clostridium tetani, Tetanus toxin (tetanospasmin, TeNT) C01394M Clostridium tetani, Tetanus toxin (tetanospasmin, TeNT) C01395M Clostridium tetani, Tetanus toxin (tetanospasmin, TeNT)
Mouse B416M EIA,WB,Neut Mouse B417M EIA,WB,Neut Mouse B418M EIA,WB,Neut
N Purified N Purified N Purified
ANTIGENS Catalog # Specificity
Host / Source
Dry Ice
Format
Preservative
R01372 R01374 R01370
Clostridium botulinum Toxin A (a.a. 1280-1292) Clostridium botulinum Toxin B (a.a. 1278-1291) Clostridium botulinum Toxin E (a.a. 2-17)
Synthetic Synthetic Synthetic
Purified Purified Purified
N None N None N None
Matched antibody pairs
Specificity
Capture Antibody
Detection Antibody
Clostridium botulinum Type A Toxoid Clostridium botulinum Type B Toxoid Clostridium botulinum Type E Toxoid
C86211M C86336M C86169M
C86468M C86018M C86042M
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Gram positive Bacteria
products
Listeria ANTIBODIES Catalog # Specificity
Host / Source Mouse Mouse Mouse Mouse Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
C86020M Listeria monocytogenes C86030M Listeria monocytogenes C86503M Listeria monocytogenes C86713M Listeria monocytogenes C86920M Listeria monocytogenes
LZA2 LZH1 LZG5 LZG7 LZF7
EIA,WB N Purified EIA,Pr,WB N Purified EIA,WB N Purified EIA,WB N Purified EIA,Pr,WB N Purified
NaN3 NaN3 NaN3 NaN3 NaN3
Matched antibody pairs
Specificity
Capture Antibody Detection Antibody
Listeria monocytogenes
C86030M
C86920M
Streptococcus ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C55560M Streptococcus agalactiae C01532M Streptococcus Group A C55504M Streptococcus Group A C55769M Streptococcus Group A C65421M Streptococcus Group A
Mouse BDI560
EIA EIA EIA EIA EIA
N Purified N Purified N Ascites N Ascites N Purified N Purified
None NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 None None
Mouse Mouse Mouse Mouse Mouse Mouse
501 504 501 042
C65043M Streptococcus Group A (carbohydrate)
3401
EIA,LF,Pr
C65721M Streptococcus Group B
072
EIA,IFA,IP N Purified
C55230M Streptococcus pneumoniae, Surface Protein A C55220M Streptococcus pneumoniae, Surface Protein A
Mouse BDI202 Mouse BDI020
EIA EIA
N Purified N Purified
Matched antibody pairs
Specificity
Capture Antibody
Detection Antibody
Streptococcus Group A
C65043M
B65150G
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Gram Positive Bacteria
products
Staphylococcus ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C01543M Staphylococcal Enterotoxin B (SEB) C01544M Staphylococcal Enterotoxin B (SEB)
Mouse B874M EIA,Pr Mouse B875M EIA,Pr
N Purified N Purified N Purified N Purified N Purified
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 None NaN3 NaN3 NaN3 NaN3 NaN3 NaN3
C55570M Staphylococcus aureus C55573M Staphylococcus aureus C55704M Staphylococcus aureus
Mouse Mouse Mouse Mouse Mouse Mouse
702 703 704
EIA EIA EIA
C86203M Staphylococcus aureus Enterotoxin C86230M Staphylococcus aureus Enterotoxin C86231M Staphylococcus aureus Enterotoxin C86104M Staphylococcus aureus Enterotoxin A (SEA) C86107M Staphylococcus aureus Enterotoxin A (SEA) C86205M Staphylococcus aureus Enterotoxin A (SEA) C86208M Staphylococcus aureus Enterotoxin A (SEA) C86411M Staphylococcus aureus Enterotoxin A (SEA) C86510M Staphylococcus aureus Enterotoxin A (SEA) C86712M Staphylococcus aureus Enterotoxin A (SEA) C14140M Staphylococcus aureus Enterotoxin B C86220M Staphylococcus aureus Enterotoxin B C86400M Staphylococcus aureus Enterotoxin B C01273M Staphylococcus aureus Enterotoxin G (SEG) C01274M Staphylococcus aureus Enterotoxin G (SEG) C01271M Staphylococcus aureus Enterotoxin I (SEI) C01272M Staphylococcus aureus Enterotoxin I (SEI)
S5 S1 S2
EIA,WB N Purified EIA,WB N Purified EIA,WB N Purified
Mouse B007M EIA,WB,Pr Mouse B002M EIA,WB,Pr Mouse B008M EIA,WB,Pr Mouse B006M EIA,WB,Pr Mouse B005M EIA,WB,Pr Mouse B003M EIA,WB,Pr Mouse B004M EIA,WB,Pr
N Purified N Purified N Purified N Purified N Purified N Purified N Purified N Purified
Mouse Mouse Mouse
SEB S222 S643
EIA
EIA,Pr,WB N Purified EIA,Pr,WB N Purified
Mouse B189M EIA,WB,Pr Mouse B191M EIA,WB,Pr Mouse B186M EIA,WB,Pr Mouse B187M EIA,WB,Pr
N Purified N Purified N Purified N Purified
Matched antibody pairs
Specificity
Capture Antibody
Detection Antibody
C86104M C86104M C86107M C86205M C86208M C86411M C86510M C86712M
C86205M C86208M C86104M C86104M C86104M C86104M C86104M C86205M
Staphylococcus aureus Enterotoxin A (SEA)
C86220M C01543M C01544M
C86400M C01544M C01543M
Staphylococcus aureus Enterotoxin B (SEB)
C01273M
C01274M
Staphylococcus aureus Enterotoxin G (SEG)
C01271M
C01272M
Staphylococcus aureus Enterotoxin I (SEI)
- 11 -
Viruses
products
Viruses Half of the reported foodborne disease outbreaks in developed countries have no identified agent and literature to date suggests that these outbreaks are caused by viruses. The Center for Disease Control and Prevention (CDC) estimated that 67% of foodborne illnesses in 1999 in the U.S. alone, were caused by a Norwalk-group of viruses (noroviruses) (Mead et al, 1999). Viruses require a host in order to multiply and human infection can occur following consumption of contaminated food, person-to-person body contact, or release of aerosols; viruses cannot grow in food. Contamination of food may occur either during preparation and serving by infected food handlers or by contact with sewage or sewage-polluted water. Viruses represent one of the most unrecognized causes of foodborne and waterborne outbreaks. Furthermore, highly pathogenic diseases which have been traditionally limited to animals are crossing the species boundary to humans, such as avian and swine influenza. This suggests the need for adequate, rapid diagnostic tools to detect viruses in food which overcome the problematic requirement for a living host or animal tissue for growth, combined with the low level of contamination in foods. Traditional methods have relied on cell culture and complex extraction methods but these techniques are not suitable for routine application and recovery rates remain poor, prompting the need for updated virus-detection procedures which are cost-effective and allow for practical implementation. News Medical. http://www.news-medical.net/news/2008/04/09/37149.aspx. (July 2012) Mead et al. (1999). Food-related illness and death in the United States. Emerging Infectious Diseases 5(5):607-625. http://www.cdc.gov/ncidod/eid/vol5no5/pdf/mead.pdf Riemann , H.P. Cliver, D.O. (2005). Foodborne Infections and Intoxications. 3d ed. Academic Press (Elsevier): London, Amsterdam. World Health Organization (2008). Viruses in Food: Scientific Advice to Support Risk Management Activities. Retrieved from http://www.who.int/foodsafety/publications/micro/Viruses_in_food_MRA.pdf
Hepatitis A ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C65868M Hepatitis A Virus (HAV) C65881M Hepatitis A Virus (HAV) C65885M Hepatitis A Virus (HAV) C86318M Hepatitis A Virus (HAV)
Mouse BDI868
EIA,IHC N Purified EIA,IFA,IHC N Purified
NaN3 NaN3 NaN3 NaN3
Mouse Mouse
818 581
EIA
N Purified
Mouse MK-01
EIA,WB N Purified
ANTIGENS Catalog # Specificity
Host / Source FRhK-4 Cells FRhK-4 Cells E. coli
Dry Ice
Tested Apps
Format
Preservative
8198
Hepatitis A Virus (HAV) Antigen, >60% Viral Protein
Partially Purified Y
NaN3
EIA,WB
Hepatitis A Virus (HAV) Antigen, Concentrate, >60% Viral Protein Hepatitis A Virus (HAV) P3C, Recombinant Hepatitis A Virus (HAV) P2C, Recombinant Hepatitis A Virus (HAV) P2C-P3A, Recombinant Hepatitis A Virus (HAV) VP1, Recombinant Hepatitis A Virus (HAV) VP1-P2A, Recombinant Hepatitis A Virus (HAV) VP3, Recombinant Hepatitis A Virus (HAV) VP4-VP2, Recombinant
8505
Partially Purified Y
NaN3
EIA,WB
R18110 R18910 R18210 R18710 R18810 R18610 R18510
Purified Purified Purified Purified Purified Purified Purified
N N N N N N N
None None None None None None None
EIA,WB EIA,WB EIA,WB EIA,WB EIA,WB EIA,WB EIA,WB
E. coli E. coli E. coli E. coli E. coli E. coli
www.MeridianLifeScience.com
- 12 -
Viruses
products
Norovirus ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C01257M Norovirus, capsid protein C01258M Norovirus, capsid protein
Mouse B113M EIA,Pr Mouse B114M EIA,Pr
N Purified N Purified
NaN3 NaN3
Matched antibody pairs
Specificity
Capture Antibody
Detection Antibody
C01257M C01258M
C01258M C01257M
Norovirus
Prion Protein ANTIBODIES Catalog # Specificity
Host / Source Mouse Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
Q06120M Prion Protein (a.a. 93-109)
6D11
EIA,IHC,WB Y EIA,IHC,WB Y
Purified Purified
None None NaN3
Q06850M Prion Protein (PrP) Q65115M Prion Protein (PrP)
7D9
Mouse BDI115 EIA,IHC,WB N Purified
Rotavirus ANTIBODIES Catalog # Specificity C65194M Rotavirus C65196M Rotavirus C65197M Rotavirus MCV41-121 Rotavirus MCV41-124 Rotavirus
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Mouse B194M EIA,IFA,Pr Mouse B196M EIA,IFA,Pr Mouse B197M EIA,IFA,Pr
N Purified N Purified N Purified
NaN3 NaN3 NaN3 NaN3 None NaN3 NaN3 NaN3 NaN3 NaN3
Mouse 213-10121 Mouse 213-15124
EIA,IFA Y EIA,IFA Y
Purified Purified
C65521M Rotavirus broad reactivity
Mouse Mouse
521 3F7
EIA,WB N Purified
C66130M Rotavirus Group A specific Antigen VP6 C01295M Rotavirus Group A Specific Antigen, VP6 C86310M Rotavirus Group Specific Antigen
WB
N Purified N Purified
Mouse B316M N/A
Mouse
3C10
EIA,WB,LF N Purified
C11222M Rotavirus NCDV (Nebraska Calf Diarrhea Virus), p43 (vp6) Mouse B260M N/A
N Purified
ANTIGENS Catalog # Specificity
Host / Source MA104 Cells
Dry Ice
Tested Apps
Format
Preservative
7844
Rotavirus Antigen (Strain SA-11) >60% Viral Protein
Partially Purified Y
None
EIA
Matched antibody pairs
Specificity
Capture Antibody Detection Antibody
C65196M C65196M
C65194M C65197M
Rotavirus
- 13 -
protozoan (waterborne)
products
Protozoan (waterborne) Over recent decades, parasitic protozoa have been recognized as having great potential to cause waterborne and foodborne disease. In the developing world, contamination of drinking water with protozoan pathogens poses a serious threat to millions of people, however, periodic outbreaks are also seen in the developing world, often caused by the protozoan parasites Cryptosporidium sp., Giardia sp., and Entamoeba histolytica. Parasite-free drinking water relies on conventional water treatment — a regimen of coagulation/flocculation, sedimentation, filtration and disinfection which normally removes protozoan parasites effectively. Simple, cheap methods for the identification of protozoans in environmental samples are still needed as the standard procedures for the detection of many species were developed several decades ago and rely on microscopy identification of cysts and trophozoites.
Bouzid, M. Steverding, D. and Tyler, K.M. (2008). Detection and surveillance of waterborne protozoan parasites Current Opinion Biotechnololg,19(3):302-6. Dawson, D. (2005). Foodborne protozoan parasites. International Journal of Food Microbiology, 103: 207– 227.
Cryptosporidium parvum ANTIBODIES Catalog # Specificity
Host / Source Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
C65760M Cryptosporidium parvum (Oocysts) Human/Bovine C01484M Cryptosporidium parvum, membrane antigen
107
EIA
N Purified
NaN3 NaN3
Mouse B621M EIA,IFA N Purified
Entamoeba histolytica ANTIBODIES Catalog # Specificity C65535M Entamoeba histolytica C65553M Entamoeba histolytica C55170M Entamoeba histolytica HK-9 C55500M Entamoeba histolytica HK-9
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Mouse BDI535 Mouse BDI553
EIA
N Purified N Purified N Ascites N Ascites
NaN3 NaN3 NaN3 NaN3
EIA,IHC(p)
Mouse Mouse
5001 5000
EIA EIA
Giardia ANTIBODIES Catalog # Specificity C01597M Giardia lamblia C01598M Giardia lamblia C65361M Giardia lamblia C65612M Giardia lamblia C65672M Giardia lamblia
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Mouse B1261M EIA,WB,IFA,Pr N Purified
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3
Mouse B1262M EIA,IFA,Pr
N Purified
Mouse BDI361 EIA,IFA,WB,Pr N Purified Mouse BDI612 EIA,IFA,WB,Pr N Purified
Mouse BDI276
EIA,IFA,Pr
N Purified N Purified
C01485M Giardia lamblia, extracellular domain
Mouse B623M IFA
Matched antibody pairs
Specificity
Capture Antibody Detection Antibody
C65672M C65672M C01598M C65672M C65612M
C65361M C01597M C65361M C65361M C65361M
Giardia lamblia
www.MeridianLifeScience.com
- 14 -
Natural toxins
products
Natural Toxins Foods can contain natural toxins as a result of product spoilage, damage and mold growth. Common plant-based toxins such as lectins (found in many types of beans), glycolalkaloids (found in potatoes), and trypsin inhibitors (found in soybeans) are not toxic in humans unless consumed in high amounts. Contamination of fish with toxins can occur from naturally occurring marine algae (phycotoxins) and many fungi and mold can excrete natural secondary toxic compounds called mycotoxins which exert harmful effects in humans and animals at very low levels. Bacteria can also produce toxins such as Vibrio cholerae, the bacterium responsible for cholera which is transmitted by the ingestion of raw or undercooked seafood and drinking contaminated water. Conventional analytical methods for the detection of toxins typically include chromatographic techniques which can be slow and expensive since they require highly-trained personnel and costly equipment. The need for simple, rapid, and cost-effective methods for detecting natural toxins that can be handled on-site, have become increasingly important to food manufacturers in order to keep commodities and products moving rapidly through the marketing channels, saving time and money. The advance of immunological techniques is becoming more useful for toxin detection due to their improved specificity and sensitivity.
Centers for Disease Control and Prevention (2012). Laboratory Methods for the Diagnosis of Vibrio cholera. Retrieved from http://www.cdc.gov/cholera/laboratory.html Miliotis, M.D., Bier, J.W. (2003). International Handbook of Foodborne Pathogens. New York: CRC Press.
Aflatoxin ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
K55980M Aflatoxin
Mouse AFA-1
EIA
N Purified
None
Aspergillus ANTIBODIES Catalog # Specificity
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
C65514M Aspergillus
Mouse BDI514
EIA
N Purified
NaN3
ANTIGENS Catalog # Specificity
Host / Source
Dry Ice
Format
Preservative
R14200
Aspergillus fumigatus
Culture Partially Purified Y
None
- 15 -
Natural toxins
products
HT-2 Toxin ANTIBODIES Catalog # Specificity C01389M HT-2 toxin C01390M HT-2 toxin
Host / Source Mouse Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
2108 2109
N/A N/A
N Purified N Purified N Purified N Purified
None None NaN3 NaN3
C01391M HT-2 toxin and T-2 toxin C01392M HT-2 toxin and T-2 toxin
Mouse B413M N/A Mouse B425M N/A
Microcystin ANTIBODIES Catalog # Specificity C01515M Microcystin-LR C01516M Microcystin-LR
Host / Source
Dry Ice
Clone # Tested Apps
Format
Preservative
Mouse B762M EIA Mouse B764M EIA
N Purified N Purified
NaN3 NaN3
Verotoxin ANTIBODIES Catalog # Specificity
Host / Source Mouse Mouse Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
C65583M Verotoxin I (Shiga-like) B subunit (SLT-1b, STX-1b) C65585M Verotoxin II (Shiga-like) A subunit (SLT-2a, STX-2a) C65582M Verotoxin II (Shiga-like) B subunit (SLT-2b, STX-2b)
357 557 257
EIA,Neut EIA,Neut EIA,Neut
N Purified N Purified N Purified
NaN3 NaN3 NaN3
www.MeridianLifeScience.com
- 16 -
ordering information
To Order Customers may order on-line at www.MeridianLifeScience.com or by fax, mail or email the purchase order. Please use the full product description, product catalog number, unit size, quantity required, and include any special instructions (i.e., lot number).
ADDRESS
TELEPHONE 901.382.8716 800.327.6299
FAX
5171 Wilfong Road Memphis, TN 38134
901.382.0027
EMAIL For Orders: orders@meridianlifescience.com For Inquiries: info@meridianlifescience.com
Payment Terms are Net 30 days and all payments must be made in U.S. dollars. Payment is accepted by the following methods, (please inquire for specific details): Check Wire transfer VISA, MasterCard and American Express Product specifications Specification Sheets are available on all products. Please call the Client Services Department or visit our website: www.MeridianLifeScience.com. Products are “For Research Use Only (RUO). Not for Use in Diagnostic Procedures.”
WHY PARTNERWITH MLS? • Unparalleled experience and proven track record (almost 30 yrs) • Core expertise in cell culture, ascites, protein production and purification • Flexibility in scale and production systems • Complete R&D and Process Development services • Robust quality system • All manufacturing conducted under ISO 9001 and cGMP • Continuous improvement through Lean Six-Sigma • Complete confidentiality
Meridian Life Science, Inc. 5171 Wilfong Road | Memphis, TN 38134 901.382.8716 • 800.327.6299
Innovative Solutions. Trusted Partner. ® Life Science, Inc. ®
August 2012
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