Meridian Food-and-Water

Capabilities

MANUFACTURING SITES

LONDON, UK PCR / qPCR Assay Development RNA Analysis DNA MW Marker Mfg

MEMPHIS,TN Viral Antigens

Recombinant Proteins In Vitro Antibody Mfg HAMA Blocking Agents Antibody Purification Contract R&D and PD Cell and Viral Banking cGMP Vaccine Mfg

BERLIN, DE Large Scale Nucleotides Mfg PCR Enzyme Mfg Protein Expression and Purification DNA MW Markers

BOCA RATON, FL Ascites Production Large Scale MAb Mfg 55,000 Mice (BALB/c, CAF1)

SYDNEY, AUS Competent Cells Mfg Diagnostic Extraction Controls

Parent Company: Meridian Bioscience, Inc. Diagnostic Test Kits | Founded in 1977; IPO in 1986 | Nasdaq: VIVO Headquartered in Cincinnati, OH | Employees: 500+ | International Presence, 60+ countries

OVERVIEW OF CAPABILITIES

PRODUCTION SYSTEMS Tissue culture flasks Spinner flasks Roller bottles (smooth and ribbed) Cell factories WAVE Bioreactors TM Fermentors

EXPRESSION SYSTEMS Mammalian (CHO, Vero, NS0, etc.) Bacterial ( E.coli ) Yeast ( P. pastoris, S. cerevisiae, etc.) Baculovirus in Sf9 Egg-based Virus Production Vaccinia & Adeno

PURIFICATION METHODS Ultrafiltration Diafiltration TFF (plate or hollow fiber)

Centrifugation/ultracentrifugation ÄKTA FPLC systems for SEC, Affinity, Ion exchange, HIC Dialysis, ultracentrifugation, tangential flow Expanded bed chromatography Centrifugation, gradient centrifugation Microfluidization, freeze/thaw, chemical lysis

QUALITY CONTROL AND ANALYTICAL TESTING Mature quality system with dedicated QA/QC and Regulatory team In-house testing (e.g. protein concentration, HPLC, ELISA, SDS-PAGE, Western blot) ISO 9001 Quality System Regulations - Applicable sections of 21 CFR 820 Current Good Manufacturing Practices - Applicable Sections of 21 CFR 210, 211

WAVE is a trademark of GE Healthcare

www.MeridianLifeScience.com

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