Gram Negative Bacteria
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Gram Negative Bacteria Gram-negative bacterial pathogens are a worldwide cause of morbidity and mortality. They are responsible for a wide variety of infections including foodborne and waterborne illness, respiratory infections, and sexually transmitted diseases. Environmental sources of the organisms include raw meat or other raw food items, juice and water. The pathogenic capability of Gram-negative bacteria is associated with a unique component of its cell envelope, in particular the lipopolysaccharide layer (also known as LPS or endotoxin) which produces host toxicity. Treating Gram-negative infections can be difficult due to this LPS layer which also serves to protect the bacteria through enabling its resistance to many drugs such as antibiotics, detergents and chemicals. The spread of antibiotic and drug resistance in these bacteria is complicating their treatment and forcing doctors to find alternative therapies or rely on outdated antibiotics that can cause kidney and nerve damage. Traditional detection methods for Gram- negative bacteria that cause foodborne and waterborne outbreaks rely on time-consuming stool cultures and the error-prone Gram stain. Alternative methods which are accurate and rapid are critical to effectively manage the treatment of outbreaks where the infectious dose can be as low as 1 –10 cells.
National Institute of Allergy and Infectious Disease (2012). Antimicrobial Drug Resistance. Retrieved from http://www.niaid.nih.gov/topics/antimicrobialresistance/examples/gramnegative/Pages/default.aspx Todar, K. Bacterial Resistance to Antibiotics. The Microbial World. (2009). July 2012. Retrieved from http://textbookofbacteriology.net/tuberculosis.html
Campylobacter jejuni ANTIBODIES Catalog # Specificity C01614M Campylobacter jejuni C65717M Campylobacter jejuni C65727M Campylobacter jejuni C86082M Campylobacter jejuni C86083M Campylobacter jejuni C86326M Campylobacter jejuni
Host / Source Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
57-24
EIA
N Dil.Sup.
NaN3 NaN3 NaN3 NaN3 NaN3 NaN3 None
Mouse BDI717 Mouse BDI727
EIA,WB N Purified EIA,WB N Purified
Mouse B082M EIA,WB N Purified Mouse B083M EIA,WB N Purified Mouse B140M EIA,WB N Purified Mouse B617M EIA,IFA N Purified
C01482M Campylobacter jejuni Types 1 & 2, non-flagellar
ANTIGENS Catalog # Specificity
Host / Source Culture
Dry Ice
Format
Preservative
R14900
Campylobacter jejuni Antigen
Purified
Y
None
Gram Negative Endotoxin ANTIBODIES Catalog # Specificity
Host / Source Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse
Dry Ice
Clone # Tested Apps
Format
Preservative
C55156M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55158M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55159M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55160M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55304M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55305M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55308M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55509M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C01575M Gram Negative Endotoxin (E. coli 0:111 B4 J5) C55157M Gram Negative Endotoxin (E. coli LPS)
301 303 306 507 304 305 308 509 305 302
EIA EIA EIA EIA EIA EIA EIA EIA EIA EIA
N Ascites N Ascites N Ascites N Ascites N Ascites N Ascites N Ascites N Ascites N Purified N Ascites
NaN3 NaN3 None None NaN3 None NaN3 None None None
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