Reverse Transcriptases (RTases)

Lyo-Compatible MMLV-RT (MDX042)

High concentration, glycerol-free reverse transcriptase ideal for ambient temperature stable molecular assays.

Features

• Glycerol free, ideal for lyophilization and air-drying applications • Reverse transcriptase activity up to 45°C • High-quality, first-strand cDNA synthesis most one-step RT-PCR and RT-qPCR applications • Sensitive detection of low copy number RNA targets

Performance Data

Higher Enzyme Efficiency and Sensitivity MMLV-RT is an unstable enzyme, so glycerol is normally added as it is an osmolyte, stabilizing the protein and preventing crystal formation during freezing, however, high glycerol concentrations are not compatible with drying down assays. The high concentration of Lyo-Compatible MMLV-RT overcomes these stability issues, allowing the glycerol to be removed and in turn allowing assays to be dried. Lyo-Compatible MMLV-RT is designed for multiplex RT-qPCR assays, allowing high sensitivity detection of low copy number RNA targets.

Hepatitis virus (RNA)

Cytomegalovirus (DNA)

The high efficiency of Lyo-compatible MMLV-RT when used with Lyo-Ready qPCR Mix (Cat# MDX021) in multiplex RT-qPCR assays with Hepatitis virus (RNA) and Cytomegalovirus (DNA) allows for high sensitivity detection of low copy number targets (as few as five viral particles).

RNase-Tolerant MMLV-RT (MDX043)

Reverse transcriptase/ RNase inhibitor mix optimized for one-step RT-qPCR.

Features

• High efficiency where samples are likely to contain inhibitors • Reverse transcriptase activity up to 50°C • Sensitive detection of low copy number RNA targets • High-quality, first-strand cDNA synthesis ideal for one-step RT-qPCR

Performance Data

High sensitivity RNase-Tolerant MMLV-RT is a 100x reverse transcriptase/RNase inhibitor mix optimized for one-step RT-PCR. RNases are ubiquitous and it is very easy to contaminate samples during reaction setup or sample preparation, even traces of RNase can be detrimental, nicking the RNA and causing shortened cDNA products, low yields, and reduced RT-PCR sensitivity. The RNase Inhibitor in RNase-Tolerant MMLV-RT is a highly efficient inhibitor of a broad spectrum of eukaryotic RNases and shows no inhibition of the reverse transcriptase or polymerase activity. It can be used with a broader temperature range, for the detection of RNA at very low levels, making it ideal for applications such as blood bank or transplant viral testing. Sensitive detection of viruses from purified blood extracts HIV-2 (5 IU/mL) HAV (5 IU/mL)

24 replicates of purified blood viral extracts were tested for HIV-2 and HAV using Low LOD 1-Step RT-qPCR Mix (Cat# MDX025) and RNase-Tolerant MMLV-RT in multiplex RT-qPCR assays. The results demonstrate the sensitivity of the RNase-Tolerant MMLV-RT, allowing high sensitivity detection of low copy number targets (as few as five viral IU/mL (international units per millilitre)).