MMLV-RT (MDX044)
Reverse transcriptase, ideal for most RT-PCR and RT-qPCR applications.
Features
• Lower RNase H activity for high yield • Reverse transcriptase activity up to 45°C • Sensitive detection of low concentrations of template RNA • High-quality, first-strand cDNA synthesis ideal for most RT-PCR and RT-qPCR applications
Performance Data
High sensitivity MMLV-RT is a 100x high-quality reverse transcriptase that synthesizes a complementary DNA (cDNA) strand from mRNA or total RNA. With higher thermostability and reduced RNase H activity, it can be used for synthesis with long messenger RNA templates with high sensitivity and efficiency and is ideal for detecting low-level target genes. Sensitive transcription, of a 1 kb fragment of calnexin from down to 10pg total RNA
A 5-fold serial dilution of total RNA (1 μg to 10 pg) was reverse transcribed using MMLV-RT, and the resultant cDNA was then used as a template in a PCR with primers for amplification of a 1 kb fragment from calnexin. The results demonstrate the high sensitivity of MMLV-RT even when the amount of template RNA is limited.
Ordering Information
PRODUCT
CAT NO.
VOLUME
REACTIONS 10,000 Units 40,000 Units 200,000 Units 2,000,000 Units 10,000,000 Units 1,000 Reactions 10,000 Reactions 1,000 Reactions
50 µ L
200 µ L
55C MMLV-RT
1 mL
MDX117
10 mL 50 mL
8 µ L
Lyo-Compatible MMLV-RT
MDX042
80 µ L
200 µ L
RNase Tolerant MMLV-RT
2 mL 10,000 Reactions 210 mL 50,000 Reactions
MDX043
200 µ L
1,000 Reactions 10,000 Reactions
MMLV-RT
MDX044
2 mL
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
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