Study 1: Speed and Reproducibility of Lyo-Ready Direct DNA LAMP Saliva (MDX134) with the ATI Gene and Viral Transport Media (VTM)
copies of MPXV DNA (over 6 repeats for each dilution). The results illustrate the greater reproducibility of the Air-Dryable™ Saliva mix, leading to increased sensitivity and specificity of MPXV detection, particularly when only low copies of the viral DNA are present.
A)
A) Lyo-Ready Direct DNA LAMP Saliva (MDX134) was lyophilized with ATI primers and used for the amplification of a 10-fold serial dilution of MPXV DNA (6,000, 600 and 60 copies respectively) in the presence of 10% VTM and 20% VTM. The time to results (TTR) values were compared to the same primers and DNA with NEB WarmStart ® and Thermo SuperScript™ IV.
B)
10% VTM- 60 copies
20% VTM- 600 copies
Lyophilized MDX134 | NEB WarmStart ® | Thermo SuperScript™ IV RT-LAMP
B) In order to illustrate the results further, the results of Lyo-Ready Direct DNA LAMP Saliva (MDX134) ( red ), NEB WarmStart ® ( orange ) and Thermo SuperScript™ IV ( black ) for the 60 copies of MPXV DNA in 10% VTM and 600 copies of MPXV DNA in 20% VTM are also displayed as amplification plots. The results illustrate the increased speed and specificity of the Lyo-Ready Direct DNA LAMP Saliva, with earlier TTR and greater reproducibility, particularly with low copies of the viral DNA.
www.meridianbioscience.com/lifescience
Powered by FlippingBook