Study 1: Speed and Reproducibility of Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) with the ATI Gene and Viral Transport Media (VTM)
of the viral DNA. The second study examined the
reproducibility of Air-Dryable™ Direct DNA qPCR Saliva against Thermo TaqPath™ ProAmp Multiplex Master Mix in a serial dilution assay using 2,000, 200 or 20 copies of MPXV DNA (over 6 repeats for each dilution). The results illustrate the greater reproducibility of the Air-Dryable™ Saliva mix, leading to increased sensitivity and specificity of MPXV detection, particularly when only low copies of the viral DNA are present.
A)
A) Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) was lyophilized with ATI primers and used for the amplification of a 10-fold serial dilution of MPXV DNA (6,000, 600 and 60 copies respectively) in the presence of 10% VTM and 20% VTM. The time to results (TTR) values were compared to the same primers and DNA with NEB WarmStart ® and Thermo SuperScript™ IV.
B)
10% VTM - 60 copies
20% VTM - 600 copies
Lyophilized MDX134 | NEB WarmStart ® | Thermo SuperScript™ IV RT-LAMP
B) In order to illustrate the results further, the results of Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) (red), NEB WarmStart ® (orange) and Thermo SuperScript™ IV (black ) for the 60 copies of MPXV DNA in 10% VTM and 600 copies of MPXV DNA in 20% VTM are also displayed as amplification plots. The results illustrate the increased speed and specificity of the Lyo-Ready™ Direct DNA LAMP Saliva, with earlier TTR and greater reproducibility, particularly with low copies of the viral DNA.
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