Efficient Amplification in Samples Containing a High Concentration of Stool
Lyo-Ready™ Direct RNA/DNA LAMP Stool (MDX145) lyophilized
Lyo-Ready ™ Direct RNA/DNA LAMP Stool (MDX145) was lyophilized and resuspended with increasing amounts of human stool (1.66 mg/mL – 19.98 mg/mL) containing inactivated RSV and primers for the RSV. Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across six technical replicates. The results illustrate that Lyo-Ready ™ Direct RNA/DNA LAMP Stool maintains a robust performance up 19.98 mg/mL stool.
20.00
15.00
10.00
5.00
0.00
0 mg/mL
1.66 mg/mL
3.33 mg/mL
6.66 mg/mL
9.99 mg/mL
13.32 mg/mL
16.65 mg/mL
19.98 mg/mL
Superior Assay Sensitivity in Challenging Environments such as Low Copy Input and High Bile Salt Concentration
A) Lyo-Ready ™ Direct DNA LAMP Stool (MDX144)
B) Lyo-Ready ™ Direct RNA/DNA LAMP Stool (MDX145)
Respiratory Syncytial Virus
Salmonella typhimurium
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20
20
10
10
0
0
1000
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10
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MDX144 | NEB WarmStart ® | Thermo SuperScript ™ IV
MDX145 | NEB WarmStart ® | Thermo SuperScript ™ IV
A) Lyo-Ready ™ Direct DNA LAMP Stool (MDX144) liquid format ( blue ), was used for the amplification of 1,000, 100 and 10 and 1 copies of Salmonella DNA in the presence of 3 mg/mL bile salts and B) Lyo-Ready ™ Direct RNA/DNA LAMP Stool (MDX145) liquid format ( blue ) was used for the amplification of Respiratory Syncytial Virus (RSV) in the presence of 0 mg/mL, 3 mg/mL and 5 mg/mL bile salts. The results were compared using the same primers and DNA/RNA template for NEB WarmStart ® ( orange ) and Thermo SuperScript ( black ). Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. The results demonstrate the high sensitivity of Lyo-Ready ™ Direct DNA LAMP Stool, even at 1 copy of DNA, and both mixes’ high tolerance to bile salt.
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