Lyophilization Beads Service We transform your assay or reagent into a bead.
Meridian offers lyophilization services that transform your molecular master mixes into a bead stable at room temperature. The beads provide convenient, ready-to-use reagents for a single assay. The use of lyo beads avoids many of the challenges that assay developers face, especially around stabilization and reagent incorporation into microfluidic cartridges and hand-held point of care (POC) readers.
Bead-based lyophilization offers a fast, and cost-effective method for preserving and stabilizing samples for long-term storage and use. Main advantages include:
Enhanced stability
Removes potential to cross-contaminate a central stock, reduces degradation through freeze-thaw cycles.
Greater flexibility
Beads can accommodate a range of precise sample volumes and sizes, making them suitable for a variety of applications such as microfluidic cartridges and POC devices.
Improved cost-effectiveness
Eliminates expensive cold chain shipping methods or refrigeration. Increases assay shelf-life, reducing scrap and waste.
www.meridianbioscience.com/lifescience
PROJECT SCOPE Our production batches range from as little as 5,000 beads for a pilot, up to as many as 10,000,000 beads per month, using multiple freeze dryers and bead dispensing automated state-of-the-art stations. Reagents can be dispensed in volumes from 5 µ L up to 200 µ L, with a variability of <3% and can be packaged into standard tubes, vials, plates and custom cartridges with precision monitoring and extensive process control. The beads weight and volume, retain long-term stability at ambient temperature and do not require expensive cold chain shipping or refrigeration during storage and so help to minimize environmental impact.
Capacity up to 10 million beads per month
Validation of correct drying conditions
Moisture content
Bead appearance
Custom packaging into tubes, cartridges,etc
Provide proof-of-concept prototype
Scale-up
Packaging
Pilot
Quality Testing
5,000 beads
Dissolution rate
Test assay sensitivity
Any of Meridian’s Lyo-Ready mixes or a client mix (provided it is glycerol-free)
Evaluate bead physical properties
READY-TO-USE MIXES AVAILABLE FOR LYOPHILIZATION Meridian has decades of experience working with extensive qPCR, RT-qPCR and LAMP assay reagents, we also offer lyo-ready enzymes, buffers and master mixes. Each mix requires unique lyophilization settings, which depend on the components of the formula, with a primary (sublimation) drying cycle under vacuum, that removes approximately 95% of the water, followed by a secondary (desorption) drying cycle at a higher temperature, to remove the remaining water molecules that we will optimize for each formulation.
Universal Master Mixes
Specimen-specific ™ Master Mixes
• Lyo-Ready ™ qPCR Mix • Lyo-Ready ™ 1-Step RT-qPCR Mix • Lyo-Ready ™ 1-Step RT-qPCR Virus Mix • Lyo-Ready ™ LAMP Mix • Lyo-Ready ™ RT-LAMP 1-Step Mix
• Lyo-Ready ™ Direct qPCR & RT-qPCR Blood • Lyo-Ready ™ Direct LAMP & RT-LAMP Blood • Lyo-Ready ™ Direct qPCR & RT-qPCR Saliva • Lyo-Ready ™ Direct LAMP & RT-LAMP Saliva • Lyo-Ready ™ Direct qPCR & RT-qPCR Urine • Lyo-Ready ™ Direct LAMP & RT-LAMP Urine • Lyo-Ready ™ Direct qPCR & RT-qPCR Stool • Lyo-Ready ™ Direct LAMP & RT-LAMP Stool
Enzymes
• Glycerol-Free Taq HS 50 U/ μ L • Aptamer Taq HS (Glycerol-Free) 50 U/ μ L
• High Conc. Glycerol-Free Bst • Lyo-compatible MMLV-RT • 55C MMLV-RT
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
Connect with us:
ISO 13485 Certified
02/23
Page 1 Page 2Powered by FlippingBook