MDX123
MDX123 vs TaqPath
Figure 2. Activity of Lyo-ready ™ Direct RNA/DNA qPCR Blood Mix (MDX123, red ) was compared in the ability to amplify a 10-fold long non-coding RNA target (MALAT-1 or PCA3) (10,000, 1,000, 100 and 10 copies respectively) in the presence of 5% DNase-treated-plasma and, compared against the activity of Quantabio ( grey ) and TaqPath ( black ). The results illustrate that the MDX123 mix shows higher level of sensitivity, end fluorescence and ability to tolerate DNase-treated plasma than suppliers.
MDX123 | Quantabio | TaqPath ™
Superior Sensitivity and Reproducibility in Samples Containing 10,000 copies to 1 copy of DNA in 10% Plasma
Figure 3. Activity of Lyo-Ready TM Genotyping Direct qPCR Blood ( blue ) was compared to KAPA Probe Force ( green ), TaqPath TM ( black ), and Type-it Fast ( light blue ) kits in a qPCR assay, using a 10-fold serial dilution DNA (10,000, 1000, 100, 10 and 1 copies respectively), in the presence of 10% plasma. The results illustrate that Lyo-Ready TM Genotyping Direct qPCR Blood has significantly higher sensitivity and reproducibility in assays detecting high or low copies of DNA template and in the presence of PCR inhibitors, resulting in tighter, more discrete, well-defined cluster.
MDX128 | KAPA Probe Force | TaqPath ™ | Type-it Fast
HPV Genotyping Directly from Swab
Human papillomavirus (HPV) Type 16 E6 - T350G mutation assay A) B) C)
D)
Homozygous WT (A) | Homozygous Mutant (C) | Heterozygous (A/C) | NTCs or undetermined
Figure 4. Human papillomavirus (HPV) type 16 and 18 has linked with high-risk to cervical, throat and many other types of cancer, an E6-T35OG mutation is used identify HPV type 16 from a throat swab in the presence of 40% UTM. A/ Lyo-Ready Genotyping Direct qPCR Urine, B/ Kapa Probe Force, C/ TaqPath and D/ Type-it Fast Kits. Homozygous allele A ( red ) and allele C ( blue ) and heterozygous allele A/C ( green ) with a NTC ( black ) and x for undetermined. Again, the results illustrate ability of Lyo-Ready Genotyping Direct qPCR Urine to form tighter, more distinct clustering and achieve accurate allelic discrimination of this clinically relevant mutation directly from universal transport media, used in viral swabs. Other suppliers of genotyping mixes show poor clustering and are not able to detect this mutation.
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