Advancing Precision Medicine Brochure

Accurate genetic analysis relies on highly sensitive and specific genotyping assays which can deliver tight fluorescent clusters and clear allele discrimination. A clear separation of fluorescent clusters reduces ambiguity which is important when trying to detect subtle differences in closely related alleles or challenging SNPs. For high-throughput assays, tight fluorescent clusters also streamline data analysis and interpretation while clear allele discrimination allows for efficient automation of genotyping calls, saving time and resources. Meridian’s genotyping mixes are optimized to provide highly specific allelic discrimination as demonstrated by excellent cluster separation, even in the presence of PCR inhibitors found in blood, urine, and stool. Furthermore, they possess excellent room- temperature stability for flexible pre- and post-PCR setup and analysis and can be used in a liquid or lyophilized format to create ambient-temperature stable assays, making them ideal for point-of-care (POC) devices. Tight fluorescent clusters and clear allele discrimination for genotyping assays Tighter Fluorescence Clusters with Clearer Allele Discrimination Compared to Other Commercially Available Mixes with Samples Containing a Range of Inhibitors Found in Blood K2-EDTA Whole Human Blood SNP differences between two strains of Epstein–Barr Virus (EBV) were tested using Lyo-Ready TM Genotyping Direct qPCR Blood Mix (MDX128), Roche Kapa Probe Force, TaqPath TM and Type-it Fast SNP Probe PCR Kits.

Allele A | Allele C | Allele A/C | NTC

Figure 5. 10% K2-EDTA whole human blood was tested with A/ Lyo-Ready TM Genotyping Direct qPCR Blood Mix, B/ Roche Kapa Probe Force, C/ TaqPath TM and D/ Type-it Fast SNP Probe PCR Kits, using EBV targets. Homozygous samples for allele A ( red ) and allele C ( blue ) and heterozygous samples for allele A/C ( green ) were compared with a NTC ( black ) and x for undetermined. The results illustrate ability of Lyo-Ready TM Genotyping Direct qPCR Blood (MDX128) to form tight clustering and so accurate allelic discrimination in the presence of whole blood unlike the other mixes.