Advancing Precision Medicine Brochure

As the burden of cancer continues to rise, point-of-care (POC) testing offers a promising solution for early cancer detection and treatment leading to better long-term patient survival. POC techniques are already used in primary care for screening patients for gastrointestinal cancer and advances in technology and translational medicine suggest that POC tests may, in the future, be able to diagnose gastrointestinal cancers at the patient’s bedside, in the outpatient clinic or even by the patient self-testing at home. Meridian’s specimen-specific mixes can be used as a liquid or dried down to create ambient-temperature stable assays, ideal for point-of-care (POC) testing. Suitable for POC oncology assays and lyophilization

Ability to Extend Shelf-life to Over 12 Months in Lyophilized Format

Homozygous A/A

Heterozygous A/C

Homozygous C/C

Liquid | Lyophilized

Figure 7. Lyo-Ready TM Genotyping Direct qPCR Blood (MDX128) was lyophilized, and the stability of the dried assays ( red ) was tested after 1 month at 37°C against fresh liquid mix ( blue ) in the presence of 5% EDTA Blood. Results suggest that Lyo-Ready TM Genotyping Direct qPCR Blood mix has a projected stability of more than 18 months at ambient temperature.

No Loss of Enzyme Performance (Sensitivity and Robustness) after Lyophilization

A) Lyo-Ready ™ Direct DNA qPCR Urine (MDX152)

B) Lyo-Ready ™ Direct RNA/DNA qPCR Urine (MDX153)

10,000 copies

1,000 copies

100 copies

10 copies

Liquid | Lyophilized

Figure 8. Activity of A) Lyo-Ready ™ Direct DNA qPCR Urine (MDX152) in lyophilized ( red ) and liquid ( blue ) format was compared in the ability to amplify a 10-fold serial dilution of synthetic DNA (10,000, 1,000, 100 and 10 copies respectively) and B) Activity of Lyo-Ready ™ Direct RNA/DNA qPCR Urine (MDX153) in lyophilized ( red ) and wet ( blue ) format was compared in the ability to amplify a 10-fold synthetic RNA target (10,000, 1,000, 100 and 10 copies respectively) in presence of 10% pooled urine. The results illustrate that the dried mix retains the ability to efficiently amplified to the same level as the wet mix with the same level of sensitivity and reproducibility for both.