Meridian Air Dryable Direct Stool

Air-Dryable Direct Stool For ambient-temperature stable assays capable of direct amplification from stool samples

Air-Dryable ™ Direct DNA qPCR and RNA/DNA qPCR Stool mixes are glycerol-free and contain optimized excipients compatible with air and oven drying. The mixes have been designed for the direct detection of DNA and RNA from stool specimens without extraction. PCR inhibitors found in stool specimens, such as bile salts, polysaccharides, hematin and catabolic substances, have posed challenges to developing assays that can directly amplify DNA or RNA. In addition, due to the high complexity and heterogeneity of fecal matter, sample preparation has traditionally been required to remove possible interfering substances such as food debris, microorganisms, desquamated epithelial cells and mucus from the specimen. Air-Dryable Direct DNA qPCR and RNA/DNA qPCR Stool mixes are designed for the direct qPCR/RT-qPCR analysis from stool samples, requiring minimal sample processing. The mix contains an optimized blend of additives to negate inhibitor effects while maintaining the quality and integrity of the patient sample. As the need for fast, non-invasive testing for gastrointestinal conditions increases, short turn-around times, higher sensitivity and a long shelf-life become important distinguishing features. If you are developing an infectious disease assay for parasites, viruses, bacteria, or a screening assay for inflammatory bowel disease or gastric or colon cancer, Air-Dryable Direct DNA qPCR and RNA/DNA qPCR Stool mixes will speed up your assay development time. The mix only requires the addition of primers and probes to create a liquid or ambient-temperature stable assay that can be dried in a laboratory oven, avoiding the need for expensive lyophilization.

Product Highlights

• Simple and easy to use • Suitable for singleplex or multiplex assays detecting RNA or DNA pathogens and cancerous material at very low titers from stool samples • Mixes can be used in a liquid or dry format, reducing the cost and complexity of developing an ambient-temperate stable assay • Compatible with a range of air-drying protocols

PRODUCT

CAT NO.

VOLUME REACTIONS

5 mL

1,000 Rxn

Air-Dryable ™ Direct DNA qPCR Stool

MDX140

50 mL

10,000 Rxn

5 mL

1,000 Rxn

Air-Dryable ™ Direct RNA/DNA qPCR Stool

MDX141

50 mL

10,000 Rxn

www.MeridianLifeScience.com

Sensitive detection in multiplex assays in the presence of stool inhibitors Air-Dryable ™ Direct DNA qPCR Stool (MDX140)

Esherichia coli 0157

Salmonella typhimurium

Campylobacter jejuni

Air-dried | Supplier K | Supplier Q

E. coli O157 (Shiga-like/Verotoxin 2 gene), Salmonella typhimurium (16S-23S rRNA gene internal transcribed spacer) and Campylobacter jejuni (16S-23S rRNA gene internal transcribed spacer) were amplified in a triplex reaction using Air-Dryable ™ Direct DNA qPCR Stool ( blue ) or mixes from supplier K ( green ) or supplier Q ( grey ) in the presence of 2 mg/mL bile salts. The results illustrate that Air-Dryable Direct DNA qPCR Stool is not affected by increased concentrations of bile, unlike supplier K and Q mixes.

Air-Dryable ™ Direct RNA/DNA qPCR Stool (MDX141)

Rotavirus

Adenovirus

Two RNA targets (Rotavirus and Norovirus) and two DNA targets (Adenovirus and Campylobacter jejuni ) were amplified in a quadruplex reaction using Air-Dryable ™ Direct RNA/DNA qPCR Stool ( blue ) or mixes from supplier T ( red ) or supplier Q ( grey ) in the presence of 1.5 mg/mL bile salts. The results illustrate that Air-Dryable Direct RNA/DNA qPCR Stool is less affected by increased concentrations of bile, unlike supplier T and Q mixes.

Norovirus

Campylobacter jejuni

Air-dried | Supplier T | Supplier Q

www.MeridianLifeScience.com

Efficient amplification of targets directly from stool using the mix in a liquid or dry assay format

Air-Dryable ™ Direct DNA qPCR Stool (MDX140)

Liquid ( black ) and air-dried ( red ) assays for human adenovirus were developed using the Air-Dryable ™ Direct DNA qPCR Stool mix. A 10-fold serial dilution (8,000, 800, 80, 8 and 0.8 ng respectively) using inactivated adenovirus (adenovirus 2 strain Adenoid 6) was carried out in the presence of 10% artificial stool. The results illustrate that the dried mix retains the same high application efficiency as the liquid mix, with the same level of sensitivity and reproducibility.

8,000 ng

80 ng 800 ng 8 ng 0.8 ng

Liquid | Air-dried

Air-Dryable ™ Direct RNA/DNA qPCR Stool (MDX141)

Liquid ( black ) and air-dried ( red ) assays for VLP-RNA Extraction Control Red (MDX068) were developed using the Air-Dryable ™ Direct RNA/DNA qPCR Stool Mix. A 10-fold serial dilution (10,000, 1,000, 100 and 10 copies respectively) assay was carried out in the presence of 5% artificial stool. The results illustrate that the dried mix maintains the same high application efficiency as the liquid mix, with the same level of sensitivity and reproducibility.

10,000 copies 100 copies 1,000 copies 10 copies

Liquid | Air-dried

Air-dried assays maintain a stable shelf-life for up to 12 months A) Air-Dryable ™ Direct DNA qPCR Stool B) Air-Dryable ™ Direct RNA/DNA qPCR Stool

Air-Dryable ™ Direct DNA qPCR Stool and Air-Dryable ™ Direct RNA/DNA qPCR Stool were air dried, and the stability of the dried assays were tested in an accelerated stability study. The air-dried assays ( red ) were incubated at 37ºC for 4 weeks and tested against fresh liquid mixes ( black ) in a (A) qPCR assay with 20% artificial stool or (B) RT-qPCR assay with mammalian RNA. The air-dried mixes retained the same activity as the liquid mixes (<0.5 Δ Ct) over time. Results suggest that the air-dried mixes are active following accelerated stability tests with a projected 12 months stability at ambient temperature.

Liquid | Air-dried

Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Fax: +1 901-333-8223 Toll Free: +1 800 327 6299

Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com

Connect with us:

ISO 13485 Certified

09/21

Page 1 Page 2 Page 3

meridianlifescience.com

Powered by