Performance Data
Fig 1 . SDS-PAGE analysis of HCV chimeric antigen SDS-PAGE was performed with a 10% Acrylamide gel, followed by staining with an InstantBlue Coomassie protein stain. Purified HCV chimeric antigen (Cat# BN1250) showed a single band with a molecular weight of 33kDa. The purity of HCV chimeric antigen was observed to be approximately >90% using ChemiDoc densitometric scanning.
2a
2b
MLS Cat# BN1250
100% R21
88% R20
80% R19
70% R18
60% R17
54% R16
50% R15
HCV Recombinant Antigen - Gold conjugated
46% R14
40% R13
34% R12
30% R11
Anti-HCV Ab (Patient’s serum)
26% R10
20% R9
16% R8
14% R7
12% R6
Anti-Human IgG
10% R5
7% R4
Membrane
5% R3
3% R2
1% R1
Fig 2. Rapid Lateral Flow Immunochromatography Test using HCV chimeric antigen BN1250 HCV Antibody Test assay was developed with two pre-coated lines, “C” Control line, and “T” Test line on the surface of the nitrocellulose membrane. Anti-Chicken IgY was coated on the control line, and anti Human IgG was coated on the test line region as per the figure (2a). The recombinant HCV chimeric antigen Cat# BN1250 was conjugated with colloidal gold particles and used as a detector for HCV antibodies. During the test, HCV antibodies in the patient’s serum sample interacted with recombinant HCV antigen conjugated with colloidal gold particles to form antibody-antigen gold particle complex. This complex migrated on the membrane via capillary action until the test line, where it was be captured by the monoclonal anti-human IgG. (2b). The visual limit of detection (LoD) as defined by the lowest dilution range on T line color to see be visible to the naked eye, was 2-9 for Cat# BN1250.
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