Sexually Transmitted Diseases (STDs)

Diagnosis Laboratory diagnosis is the only way to confirm an HIV infection and there are specific serologic markers that can be detected in the early course of an infection. HIV RNA: detectable by current molecular methods at about 11 days from the time of HIV infection

HIV P24 ANTIGEN: detectable 16 days from the time of infection HIV ANTIBODIES: detectable 22 days from the time of infection

HIV MARKERS DURING EARLY INFECTION

During the early infection stage (acute retroviral syndrome) the flu-like symptoms are accompanied by a burst of viral replication that can be detected in the blood. The detection of p24 antigen (viral capsid protein) is directly correlated to the amount of virus (viral load) circulating in the infected individual. Antibodies against specific HIV proteins and glycoproteins (e.g. p24, gp41, gp120) are produced between 2-8 weeks after infection and remain detectable in the blood thereafter. The screening test most widely used to detect exposure to HIV is the “HIV Antibody Test”. The first test was approved in 1985 by the FDA and it still remains one of the WHO recommended HIV diagnostics. Advances in technologies and critical reagents have enabled the development of new generation HIV Antibody Tests, which are able to detect an infected individual earlier and with greater accuracy. The 4th generation HIV Antibody Test is capable of diagnosing an HIV infection 3-4 weeks after transmission by

Source: mayomedicallaboratories.com

simultaneously detecting both HIV antibody and p24 antigen. In addition, many of these tests can also distinguish between acute and established HIV infections, as well as detect antibodies to HIV groups M and O, and HIV-2. The commercial HIV diagnostic testing market has expanded to include several testing formats such as Western blot, immunofluorescence (IFA), and lateral flow as well as various sample types such as saliva, urine, and nucleic acids. Regardless of the type of screening test used, a positive result requires follow up with a second test to confirm a diagnosis of HIV.

Reagents for serology testing

HIV-1 p24 Antibodies An early marker of infectivity. The detection of p24 antigen directly correlates to the amount of virus in an infected individual.

C01653M

MAb to HIV-1 p24 • Capture or Detection antibody MAb to HIV-1 p24 • Capture or Detection antibody

Paired MAbs for Sandwich ELISA, Lateral Flow and CLIA Antigen Detection Assays

C65690M

C01653M

MAb to HIV-1 p24 • Isotype: IgG1 • Capture antibody or Detection Antibody MAb to HIV-1 p24 • Isotype: IgG1 • Capture antibody or Detection Antibody

Paired MAbs for Sandwich ELISA and Lateral Flow Antigen Detection Assays

C01655M