Quicker time-to-results and better sensitivity
With lower sample input
The average time to results (TTR) for a Dengue Type 2 RNA target was compared using Lyo-Ready ™ RT-LAMP 1-Step Mix ( orange ) and a mix from supplier N ( blue ) on a serial dilution of Dengue Type 2 crude extract. The results demonstrate the increased sensitivity and greater reproducibility of the Lyo-Ready ™ RT-LAMP 1-Step Mix, with earlier TTR (faster) and a lower standard deviation when compared to supplier N.
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MDX097 | Supplier N
MDX108
Supplier N
And in the presence of inhibitors A Dengue Type 2 RNA target was amplified from a 1:10 dilution of Dengue Type 2 crude extract in the presence of different concentrations of a (A) UTM and artificial sputum and (B) artificial saliva using Lyo-Ready ™ RT-LAMP 1-Step Mix ( orange ) and supplier N ( blue ). Reactions were performed at 65°C for 60 min in 4 replicates. Time-to-result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. The results illustrate the faster average time-to- result (TTR) with the Lyo-Ready ™ RT-LAMP 1-Step Mix, demonstrating faster detection without inhibitors and less inhibition at high concentrations of both UTM and sputum and saliva compared to supplier N mix.
A) UTM and sputum
B) Saliva
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0% UTM + Sputum
10% UTM + Sputum
20% UTM + Sputum
0% Saliva
20% Saliva
25% Saliva
MDX097 | Supplier N
Mix performance is not impacted by lyophilization or subsequent room temperature storage
LAMP reactions in the presence ( green ) or absence ( teal ) of 10% UTM and sputum were performed in 5 replicates using PMSD4.1 primers and RNA to compare the performance of fresh (wet) Lyo-Ready ™ RT-LAMP 1-Step Mix against lyophilized Lyo-Ready ™ RT-LAMP 1-Step Mix, stored at 37ºC for 3 weeks. Time-to-result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. The data illustrate that the lyophilization process does not affect the stability of the Lyo-Ready ™ RT-LAMP Mix and at a storage temperature of 37ºC it can be extrapolated that the lyophilized mix is stable at room temperature for up to 12 months.
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Lyophilized
Wet Mix
Lyophilized
Wet Mix
No Inhibitor
10% UTM + Sputum
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