Viral Hepatitis

REAGENTS FOR SEROLOGY TESTING (Continued)

Anti-HBs

Used to determine immunity status. Used to detect previous exposure to HBV or to establish vaccination status. Subtypes adw, ady, and adr are each found in different geographic regions of the world. In Japan, China, and Southeast Asia, the r determinant predominates and in Europe, the US, Africa, India, Australia, and Oceania, the w determinant predominates. The y determinant is found in Africa and in Australia aborigines. (WHO, 2014)

R86872

Recombinant HBsAg (adw) • Comprises the full-length of the S-gene (227 a.a.) • Produced in S. cerevisiae , no fusion partner/tag, >98% pure • Molecular weight of 24kDa • Buffer: 0.05 M Phosphate, 0.2 M NaCl, pH 7.2 • Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies Recombinant HBsAg (ayw) • Produced in S. cerevisiae , no fusion partner/tag, >98% pure • Molecular weight of 24kDa • Buffer: 0.05 M Phosphate, 0.2 M NaCl, pH 7.2 • Tested as solid-phase coating antigen in ELISA with anti-HBsAg antibodies Recombinant HBsAg (adw) • Produced in S. cerevisiae , no fusion partner/tag, >95% pure • Molecular weight of 24kDa • Buffer: PBS, pH 7.2 Recombinant HBsAg (adr) • Produced in CHO cells >95% pure • Molecular weight of 23-27kDa • Buffer: 20 mM Phosphate, 154 mM NaCl, pH 7.2 ± 0.2

R86870

R01652

Suitable for ELISA Antibody Detection Assays

R01289

R01290

Recombinant HBsAg (ayw) • Produced in S. cerevisiae , no fusion partner/tag, 95% pure • Molecular weight of 24 kDa • Buffer: PBS, pH 7.2

R01403

Native HBsAg (ad) • Purified from human blood, > 99% pure (“ad” subtype purity) • Buffer: 50 mM Phosphate, pH 7.4 ± 0.2 containing 150 mM NaCl Native HBsAg (ay) • Purified from human blood, ≥ 99% pure (“ay” subtype purity) • Buffer: 50 mM Phosphate, pH 7.4 ± 0.2 containing 150 mM NaCl Recombinant HBsAg (ayw) • Produced in Pichia pastoris, no fusion partner/tag • Molecular weight of 24kDa • Buffer: 20 mM NaHCO 3 , pH 9.3, 100 mM NaCl, 3 mM EDTA, 15% Glycerol

R01507

R01508