Antibody and Antigen Detection Assays Hepatitis C (HCV)
REAGENTS FOR SEROLOGY TESTING (Continued)
HCV NS4 Recombinant Antigens
R18541
• A chimeric constructed from fragments of the NS4 immunodominant region of 11 different HCV genotypes, fused with a GST tag • Produced in E. coli (>95% pure) • Molecular weight of 66kDa • Buffer: 8 M Urea, 20 mM TRIS, pH 9.0 containing 10 mM DTT, 1 mM EDTA and 0.5% Tween-20 • Represents residues 1658-1863 of NS4a+b, fused with a -gal tag at the N-terminus • Produced in E. coli (>95% pure) • Molecular weight of 19kDa, plus 114kDa ( -gal) • Buffer: 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM -Mercaptoethanol • Represents a.a. 2322-2423 of NS5 genotype 1a, fused with a GST tag at the N-terminus • Produced in E. coli (>95% pure) • Molecular weight of 37.9kDa • Buffer: 50 mM Tris-HCl, 5 mM EDTA, pH 8.0 • Represents a.a. 2212-2313 of NS5, genotype 1, fused with a GST tag • Produced in E. coli (>95% pure) • Buffer: 50 mM Tris-HCl, 5 mM EDTA, pH 8.0
R8A116
Suitable for ELISA and Western Blot Antibody Detection Assays
HCV NS5 Recombinant Antigens
R18206
R18190
R18523 HCV Chimeric Core/NS3/NS4/NS5 Recombinant Antigens
• Represents immunodominat regions of HCV core, NS3, NS4 and NS5 • Produced in E. coli (>95% pure) • Molecular weight of 70.8kDa • Buffer: 50 mM Sodium Phosphate, pH 8.5 containing 2.4 mM EDTA, 5 mM DTT, 0.1% SDS
HCV Chimeric Core/NS3/NS4/NS5 Recombinant Antigens
R01599
• Contains four antigens expressed in E. coli in a chimera: HCV Core Fragment (a.a. 11-45), HCV NS3 Fragment (a.a. 1208-1430), HCV NS4 Fragment (a.a. 1885-1923), HCV NS5 Fragment (a.a. 2332-2423) • Produced in E. coli (>90% pure) • Molecular weight of 33kDa • Buffer: 20 mM phosphate, pH 7.4 • Contains four antigens expressed in E. coli in a chimera: HCV Core Fragment (a.a. 11-53), HCV NS3 Fragment (a.a. 1210-1450, 1337-1483), HCV NS4 Fragment (a.a. 1888-1925), HCV NS5 Fragment (a.a. 2332-2423) • Produced in E. coli (>90% pure) • Molecular weight of 71kDa • Buffer: 20 mM phosphate, pH 7.4
Antigen Pair for Lateral Flow Antibody Detection Assays
R01600
17
Hepatitis- Reagents for Assay Development
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