Hepatitis E (HEV) Antibody Detection Assays
REAGENTS FOR SEROLOGY TESTING
Tests for HEV generally detect both IgM and IgG. Studies have shown that assays based on open reading frame 2 (ORF2) can be more sensitive in detecting anti-HEV than those based on ORF3. Overall recombinant- protein-based tests have demonstrated an ability to detect anti-HEV in 90 to 95% of symptomatic HEV cases. HEV Antibody Test
R18250
HEV ORF2 and ORF3 Recombinant Antigen • Contains the HEV immunodominant regions from ORF2 and ORF3 • Immunoreactive with HEV positive sera • Produced in E. coli • Buffer: 25mM Tris-HCl, 1mM EDTA, 0.5M urea containing 50% glycerol
R8A310
HEV ORF2 Recombinant Antigen • Represents amino acids 633-659 of ORF2 • Immunoreactive with HEV positive sera • Produced in E. coli • Buffer: 1 X Phosphate Buffered Saline, pH 7.2
Suitable for ELISA Antibody Detection Assays
R8A330
HEV ORF2 Recombinant Antigen • Represents amino acids 403-461 of ORF2 • Immunoreactive with HEV positive sera • Produced in E. coli • Buffer: 8M Urea, 20mM Tris-HCl, pH 8.0 containing 10 mM -mercaptoethanol HEV ORF3 Recombinant Antigen • Represents amino acids 92-123 of ORF3 • Immunoreactive with HEV positive sera • Produced in E. coli • Buffer: 8M Urea, 20mM Tris-HCl, pH 8.0 containing 10 mM -mercaptoethanol
R8A320
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Hepatitis- Reagents for Assay Development
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