Isothermal amplification has revolutionized the point-of-care diagnostic industry, paving the way for quicker and more accessible diagnoses.
At Meridian Bioscience, we are dedicated to the development of highly sensitive, specific, and fast enzymes and master mixes to support the next generation of sensitive and affordable POC and high throughput MDx tests.
Isothermal amplification (including HAD, MCA, NASBA, and LAMP) is a DNA amplification technique that has played an increasingly important role in molecular diagnostics. It offers some advantages over qPCR in terms of sample-to-answer time and compatibility with low energy consuming devices, making it ideal for field-deployable diagnostics. Isothermal amplification assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, GM crop contamination, and forensics to detect human DNA. Isothermal Amplification Top 4 critical features in isothermal amplification that impact assay performance
• SPEED: Reduces time-to-results (TTR) and increases assay throughput • SENSITIVITY: Enables amplification of low copy-DNA or RNA templates
Loop-mediated isothermal amplification (LAMP) is one of the most common isothermal amplification methods used. However, alternative techniques such as nucleic acid sequence-based amplification (NASBA) is gaining popularity as it has the advantage of directly amplifying RNA at a lower temperature (typically around 41°C) without the need for a thermocycler or a separate reverse transcription step. Unlike LAMP which requires 4 to 6 primers, NASBA uses only two primers, which makes its primer design less complex and easier to optimize. Meridian’s LAMP and NASBA enzyme and master mixes provide state-of-the-art solutions for isothermal amplification, advancing molecular assay development. • TOLERANCE TO INHIBITORS: Enzymes with high tolerance to PCR inhibitors, improving assay robustness and reliability . • COMPATIBILITY: Assay efficiency and sensitivity is maximized when assay components such as buffer systems are optimized. For compatibility with lyophilization, the chemistry needs to be carefully optimized under glycerol-free conditions, taking into account excipients and stabilizers while ensuring no loss of performance.
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