Isothermal Amplification Brochure

LAMP

A powerful method for rapidly amplifying DNA with proven results

Loop mediated isothermal amplification (LAMP), uses four to six primers that recognize several specific regions in the target DNA. The reaction is initiated by a strand-displacement polymerase, such as Bst DNA polymerase and two specially designed primers, which form loop structures to facilitate subsequent rounds of amplification, producing high levels of DNA. LAMP reactions are very robust and inhibitor-tolerant, producing extremely large amounts of DNA that can be visualized by the eye (e.g., as a precipitate or color change). Specifically, 1 to 10 copies of DNA can be amplified to 10 9 to 10 10 copies under one hour, producing assays with excellent sensitivity and specificity.

Forward internal primer (FIP)

LAMP Reaction Workflow

*RT-LAMP requires an inital reverse transciption step to generate DNA from teh RNA template

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Backward internal primer (BIP)

LAMP is ideal for detection of pathogens (bacteria, viruses & parasites) and genetic mutations; RT-LAMP is also used for viral RNA detection. ”

Meridian’s Bst Enzyme & LAMP Master Mix Solutions

Meridian’s Bst polymerases are designed for isothermal amplification assays and are supplied as stand-alone enzymes or pre-optimized in master mixes, for the ultimate flexibility in assay design. Our glycerol-free formulation, combined with our inhibitor tolerant buffer, is specifically suited for increasing assay robustness or establishing a direct detection workflow, avoiding the need for nucleic acid extraction. For even greater tolerance and ease of assay development, Meridian developed Specimen-specific TM inhibitor tolerant LAMP master mixes, designed to enable direct amplification from crude lysates or inhibitor-rich samples. In addition, these mixes can be used as wet mixes or dried by lyophilization or air-drying to create room-temperature stable assays. Utilizing a dried assay format not only suits POC applications but also maximizes the volume of sample to be analyzed, increasing the LOD of the test. Meridian’s innovative and ready-to-use LAMP master mixes facilitate assay chemistry optimization and overall accelerate assay development. They are ideal for developing ultra-sensitive, rapid, and simple assays, perfect for increasing a test’s competitive edge and capturing value faster in the rapidly growing POC market.