Isothermal Amplification Brochure

LAMP

SPEED: Superior specificity and quicker time-to- results (TTR) detection for viral and bacterial targets

SENSITIVITY: Superior assay sensitivity and reproducibility with lower TTR and delayed NTC

60

60

50

50

40

40

30

30

20

20

10

10

0

0

Sample

NTC

Sample

NTC

Sample

NTC

Sample

NTC

Sample

NTC

Sample

NTC

Sample

NTC

Sample

NTC

Sample

NTC

Sample

NTC

HBV

HSV2

EBV

T. vaginalis

M. pneumoniae

HCV

HIV-1

DENGUE 1

WNV

Zika

Meridian Lyo-Ready Direct DNA LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™

INHIBITOR TOLERANCE: High inhibitor tolerance and fast amplification to deliver quicker time-to- results (TTR) in inhibitor-rich samples. SuperScript ™ IV RT-LAMP Master Mix ( yellow ) using the same primers and DNA/RNA for five different targets as shown in the graph. Error bars represent the standard deviation across three technical replicates. For more detailed information, refer to the product flyer. The performance of Lyo-Ready Direct LAMP Blood Mix (MDX124, blue ) were compared against NEB WarmStart ™ LAMP kit ( grey ) and Thermo

The performance of Lyo-Ready Direct LAMP Urine Mix (MDX154, Blue ) were compared against NEB WarmStart ™ LAMP kit ( grey ) and Thermo SuperScript ™ IV RT-LAMP Master Mix ( yellow ) using the same primers and DNA/RNA for five different targets as shown in the graph. Error bars represent the standard deviation across three technical replicates. For more detailed information, refer to the product flyer.

COMPATIBILTIY: Compatible with lyophilization without any loss in sensitivity and speed

ADV

HSV2

50

70 60 50 40 30 20 10 0

70 60 50 40 30 20 10 0

40

30

20

10

0

1000 copies/rxn

500 copies/rxn

100 copies/rxn 10 copies/rxn

0% inhibitor

30% saliva

30% UTM+ Sputum

0% inhibitor

30% saliva

30% UTM+ Sputum

30% VTM

NTC

30% VTM

NTC

Meridian Lyophilized | NEB WarmStart®

Meridian Lyophilized | Meridian Air-dried | NEB WarmStart®

Lyophilized Lyo-Ready Direct DNA LAMP Saliva (MDX134, blue ) was compared to liquid NEB WarmStart ™ ( orange ) for their inhibitor tolerance to a range of inhibitors in amplification reactions detecting inactivated Adenovirus (ADV) or Herpes simplex virus 2 (HSV-2). Error bars represent the standard deviation across four technical replicates. For more detailed information, refer to the product information sheet.

1,000, 500, 100 and 10 copies of Emesvirus (MS2) were amplified using Air- Dryable ™ RNA/DNA LAMP (MDX118) in an air-dried ( red ) format, liquid ( blue ) format, or using NEB WarmStart ™ ( orange ) using the same primers and probes. Error bars represent standard deviation over 4 technical replicates. Fore more detailed information, refer to the product flyer.

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