Isothermal Amplification Brochure

NASBA

A preferred choice for easy isothermal assay development, lower temperature cycling, and detection of low concentrations of RNA

NASBA is an isothermal amplification method that is able to amplify and detect RNA. This technique is particularly useful in clinical virology due to its rapid amplification, high sensitivity, and high specificity, but also in POC applications where portability is critical . NASBA utilizes two primers, known as the “sense” and “antisense” primers that are designed to target a region of interest. One of these primers includes the promoter sequence for T7 RNA polymerase. The RNA is reverse transcribed to cDNA. The RNA in the cDNA-RNA hybrid is destroyed by RNase H activity and dsDNA is produced by the reverse transcriptase. This template then gets transcribed to RNA by T7 RNA polymerase and exponential amplification results.

NASBA Reaction Workflow

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NASBA is great for highly specific amplification direcly from RNA targets at the constant and lower temprature, ideal for detecting very low concentrations of RNA is crucial, such as in viral load quantification or early detection of RNA viruses ”