NASBA
Meridian’s NASBA Enzyme Solutions Meridian’s NASBA enzymes are designed for isothermal amplification assays and are supplied at high concentrations for flexibility in assay design. Our glycerol-free formulations offer superior performance and provide the added capability of lyophilization, granting users the flexibility to store reaction mixtures at room temperature or produce diagnostic assays with miniaturized reaction components, making them specifically suited to microfluidic or cartridge based assays. NASBA reactions consist of a reverse transcriptase, T7 RNA polymerase, and RNase H with two oligonucleotide primers. Molecular beacon probes can be used for detection as they are highly specific to their target RNA and form a stable hybrid. Although NASBA can only detect one target at a time, the technique is extremely sensitive, and ideal for clinical diagnostics, particularly for infectious diseases.
SPEED: High specificity of detection with Meridian’s NASBA enzymes under 1 hour with no detectable NTCs
HIV-1 RNA
Primers and a molecular beacon probe were designed for HIV-1 RNA and used in a nucleic acid sequence-based amplification (NASBA) reaction using Glycerol-Free T7 RNA Polymerase (HC) (MDX201), Glycerol-Free RNase H (HC) (MDX202) and AMV reverse transcriptase, using 1,000 copies of an HIV-1 RNA target.
HIV-1 RNA | NTC
SENSITIVITY: Sensitive detection with Meridian’s NASBA enzymes down to 10 copies of RNA under 1 hour
SARS-CoV 2 RNA
HIV-1 RNA
0 10 20 30 40 50
0 10 20 30 40
1000 copies 100 copies 1000 copies 100 copies
10 copies 10 copies
1000 copies 100 copies 1000 copies 100 copies
10 copies 10 copies
Primers and a molecular beacon probe were designed for HIV-1 RNA and SARS COV-2 RNA and used in NASBA reactions, with a 1,000 copy, 100 copy and 10 copy serial dilution of using of the RNA targets. The results demonstrate the high sensitivity of NASBA, being able to detect down to 10 copies per reaction in under 1 hour. This detection is highly specific sensitive and consistent, making NASBA ideal technology for the next generation of molecular point of care testing assay design.
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