Mouse-FREE IgG (Recombinant, Animal-Free HAMA & HA Blocker)
Meridian Bioscience’s Mouse-FREE IgG is a HAMA blocker made from a mixture of full-length recombinant antibodies identified in native mouse IgG (IgG1, IgG2a, IgG2b, IgG3). Mouse-FREE IgG is a direct replacement to the animal version (native mouse IgG) and it has the same HAMA blocking efficiency (Fig 1).
Mouse-FREE IgG Cat# BN1300
HAMA Blocking Effectiveness - Meridian Mouse-FREE IgG vs. Native Mouse Serum
Blocking antibodies such as mouse IgG neutralize the interfering antibodies present in serum samples resulting in accurate binding of the analyte of interest by the capture and detection antibodies. Human HAMA-positive serum was incubated with Meridian’s Mouse-FREE IgG (Cat No. BN1300) or serum-derived purified native mouse IgG at concentrations ranging from 0.0001-1.25 μ g/mL for 20 mins at room temperature. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA signal – the lower EC50 values indicate greater interference suppression.
Mouse IgG
0 0.005 0.01 0.015 0.02 0.025 0.03 0.035
Mouse IgG
Interfering HA (e.g. HAMA)
IgM RF
Detection Antibody (mouse origin)
Antigen
Blocking Buffer
Capture Antibody (mouse origin)
Meridian Mouse-FREE IgG
Native Mouse IgG
A) HAMA Interference
100
100
100
80
80
69
66
60
60
42
40
40
20
20
0
0
Native Mouse IgG
Competitor Blocker #1
Competitor Blocker #2
Meridian Mouse-Free IgG
0.0001
0.001
0.01
0.1
1
10
Blocker concentration ( µ g/ml)
B) RF Interference
100
100
100
80
80
60
60
40
40
20
20
14
14
11
0
0
0.01
0.1
1
10
100
Meridian Mouse-Free IgG
Native Mouse IgG
Competitor Blocker #1
Competitor Blocker #2
Blocker concentration ( µ g/ml)
Meridian Mouse-Free IgG | Native mouse IgG | Competitor Blocker #1 | Competitor Blocker #2
Fig 1. A double mouse monoclonal sandwich assay was used to compare the performance of Meridian’s Mouse-FREE IgG (100% animal-free, recombinant Mouse IgG, Cat No. BN1300) against serum-derived purified native mouse IgG and two competitor HAMA blockers. (A) Human HAMA-positive or (B) RF-positive serum was preincubated with the individual blockers for 20 minutes at room temperature. Blocker concentrations ranged from 0.0003-1.25 μ g/mL for the HAMA assay and 0.0195-80 μ g/mL for the RF assay. The effectiveness of each blocker was determined by comparing the relative suppression of the HAMA or RF signal – lower EC50 values indicate greater interference suppression. The results demonstrate that Mouse-Free IgG is significantly more effective than native mouse IgG and the two competitor blockers at blocking HAMA and RF interference.
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