Progesterone (P4) belongs to a group of steroid hormones called the progestogens and it is the major progestogen in the body. Its main role is to maintain pregnancy and to regulate the female’s menstrual cycle. Its levels increases sharply during the luteal phase of the menstrual cycle as it works to thicken the lining of the uterus to prepare it for a fertilized egg each month. In the event that an egg does implant, progesterone’s function is to maintain the uterine lining throughout the pregnancy and the levels increase from 9 to 32 weeks. If no fertilized egg implants, progesterone levels drop and menstruation begins. Progesterone is also necessary for breast development and breastfeeding and complements some effects of estrogen. In men it works with testosterone to help in sperm development. Progesterone diagnostic assays are useful to help determine the cause of infertility, track ovulation, help diagnose an ectopic or failing pregnancy, monitor the health of a pregnancy, monitor progesterone replacement therapy, and help diagnose the cause of abnormal uterine bleeding. A related progestogen steroid hormone is 17-Hydroxyprogesterone (17-OH P) which is synthesized from progesterone and 17-Hydroxypregnenolone. It functions as a precursor of cortisol in the adrenal glands or can be converted into androgenic and estrogenic hormones. Measurement of 17-OH P is used as an aid in the diagnosis and treatment of various disorders of the adrenal glands or the ovaries. It is also part of newborn screening in many countries used to detect congenital adrenal hyperplasia (CAH). Progesterone assays are usually quantitative and based on the competitive immunoassay format.
Reagents for Immunoassay Development
MAb to Progesterone Cross-reactivity: 1 μg cortisol = 5 ng 10 ng estradiol < 5 ng
500 ng estriol < 5 ng 20 ng testosterone < 5 ng
MAb to Progesterone • Cross-reactive with 17-Hydroxyprogesterone (10%) and Deoxycorticosterone (1%) MAb to Progesterone • Cross-reactive with 17-Hydroxyprogesterone (1%) and Deoxycorticosterone (1%), 11-Hydroxyprogesterone (25%), Deoxycortisol (0.03%), 5-alpha-pregnane-3,20 dionene (10.5%), Cortisol (0.002%), Corticosterone (0.01%) Progesterone 3-HRP Conjugate • Progesterone antigen linked at the 3 position with HRP • MW ~ 45 kDa • Buffer: Tris, pH 7.6 containing protein stabilizers and 0.005% Thimerosal
Suitable for use in competitive ELISA
Endocrine Disorders - Reagents for Assay Development
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