Blockers Practical Guide

Recommended Blockers

An isotype control acts as a negative control in flow cytometry and immunohistochemistry assays by differentiating non-specific background signal from specific antibody signal. In addition, Human Immunoglobulin can be used to measure the type of immunoglobulin/ isotype in a patient’s sample, as a standard blocking agent, or as a protein coating agent for applications including immunofluorescence, immunocytochemistry, Western blot, and ELISA. Isotype Controls & Other Blockers

RECOMMENDED REAGENT: Human IgG • A50170H, IgG • A50175H, IgG (Fc) IgA • A50167H, IgA

Mouse IgG • A66186M, IgG • A01696M, IgG2a, kappa

Rat IgG

IgM • A50168H, IgM IgE • A50384H, IgE • A75671H, IgE, kappa & lambda

• A64391R, IgG • A41182R, IgG

Sheep IgG • A66400S, IgG

• A50166H, IgA1, kappa • A50165H, IgA1, lambda

Isotype controls are primary antibodies that lack specificity to the target antigen, but match the class and type of the primary antibody used in the application. They act to reduce non-specific binding by the primary/capture antibody through blocking Fc receptor binding or nonspecific antibody interactions with cellular proteins, carbohydrates, and lipids. The isotype control antibody should match the primary/capture antibody’s host species, isotype (including heavy chain (IgA, IgG, IgD, IgE, or IgM) and light chain (kappa or lambda) class) and if relevant, conjugation. In addition, isotype controls should be used at the same concentration as the primary antibody.


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