Meridian Specimen-specific qPCR & LAMP Master Mixes

Superior reaction efficiency using crude urine samples in multiplex reactions

Lyo-Ready™ Direct DNA qPCR Urine (MDX152)

Mycoplasma genitalium

Treponema pallidum

Chlamydia trachomatis

Neisseria gonorrheae

MDX152 | Quantabio ToughMix | Roche KAPA | ThermoFisher TaqPath ™

Four sexually transmitted pathogens ( M. genitalium, T. pallidum (Syphilis), C. trachomatis and N. gonorrhoeae ) were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready ™ Direct DNA qPCR Urine in a lyophilized format ( red ) and compared to Quantabio ToughMix PCR Master Mix ( grey ), Roche KAPA Probe Force qPCR kit ( green ) and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ) in a liquid format. The results demonstrate that Lyo-Ready ™ Direct DNA qPCR Urine has higher multiplexing capabilities and better reproducibility than other supplier qPCR mixes.

High amplification sensitivity with low copy templates using either liquid and dried-down assay formats

A) Lyo-Ready Direct DNA qPCR Urine (MDX152)

B) Lyo-Ready Direct RNA/DNA qPCR Urine (MDX153)

10,000 copies

1,000 copies

100 copies

10 copies

Lyophilized | Liquid

Activity of A) Lyo-Ready ™ Direct DNA qPCR Urine in lyophilized ( red ) and liquid ( blue ) format was compared in the ability to amplify a 10-fold serial dilution of synthetic DNA (10,000, 1,000, 100 and 10 copies respectively) and B) Activity of Lyo-Ready ™ Direct RNA/DNA qPCR Urine in lyophilized ( red ) and wet ( blue ) format was compared in the ability to amplify a 10-fold synthetic RNA target (10,000, 1,000, 100 and 10 copies respectively) in the presence of 10% pooled human urine. The results illustrate that the dried mix retains the ability to efficiently amplified to the same level as the wet mix with the same level of sensitivity and reproducibility for both.