Specimen-specific ™ LAMP & RT-LAMP Mixes
Blood-specific Mixes
Blood
Blood is one of the most common specimens used for laboratory diagnostic testing and is useful for evaluating the function of vital organs and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders including diabetes. Bacterial cell culture is regarded as the gold standard for microbial detection from blood as molecular methods to-date have lacked the sensitivity to detect bacteria at low concentrations due to the presence of assay inhibitors among other factors.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Blood Mix
MDX124
Lyo-Ready Direct RNA/DNA LAMP Blood Mix
MDX125
Meridian’s Lyo-Ready TM Direct DNA and DNA/RNA LAMP Blood Mixes are the newest solution for assays requiring high sensitivity and fast time to results and can detect DNA or RNA down to single copies in blood, serum or plasma samples. The mixes are designed to be extremely inhibitor-tolerant, enabling direct detection using crudely processed samples, making them ideal for developing next-generation, culture-free assays detecting bacterial infections such as sepsis. They outperform other commercial LAMP mixes in terms of sensitivity, specificity and speed which is demonstrated by their quicker time-to-result (TTR) and lower non-specific amplification (NTC) across a range of targets. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready TM Direct DNA LAMP or RNA/DNA LAMP Blood mix, assay development time for a new ultra-sensitive direct blood assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
Superior Specificity and Quicker Time to Detection for Viral and Bacterial Targets
A) Lyo-Ready™ DNA LAMP Blood (MDX124)
B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
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HBV
HSV2
EBV
T. vaginalis
M. pneumoniae
HCV
HIV-1
DENGUE 1
WNV
Zika
Lyo-Ready ™ Direct DNA LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™
(A) Lyo-Ready ™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for Trichomonas vaginalis , Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Epstein-Barr virus (EBV), Mycoplasma pneumoniae and (B) Lyo-Ready ™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for Hepatitis C Virus (HCV), HIV-1, Dengue type 1, West Nile Virus (WNV) or Zika. The performance of the mixes were compared against NEB WarmStart ® LAMP kit ( orange ) and Thermo SuperScript ™ IV RT-LAMP Master Mix (black) using the same primers and DNA/ RNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo-Ready ™ Direct LAMP Blood mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across range of pathogenic DNA and viral RNA targets.
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