Results Delivered in Half the Time with Samples Containing 20-30% Urine
Dengue Type-1 + 20% Urine
Lyo-Ready RNA/DNA LAMP Urine (MDX155)
Chikungunya + 5% Urine
CHIKV + 5% Urine
HCV + 5% Urine
ZIKV + 20% Urine
WNV + 5% Urine
DENV + 20% Urine
Lyophilized | NEB WarmStart® | SuperScript™
Lyo-Ready Direct RNA/DNA LAMP Urine (MDX155, red ) was lyophilized with primers for either Chikungunya Virus (CHIKV), Hepatitis C Virus (HCV), Zika, West Nile Virus (WNV) or Dengue Type-1. The performance of the mixes was compared against liquid formats of NEB WarmStart® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that MDX155 has earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready Direct RNA/DNA LAMP Urine (MDX155) performs better and with faster amplification across the range of inhibitors compared to the other mixes.
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