Maintains sensitive amplification using fast cycling conditions
Standard cycling (54 min)
Fast cycling (37 min)
A
B
The performance of Lyo-Ready ™ Direct DNA qPCR Blood (MDX122) on two mammalian DNA targets and Lyo-Ready ™ Direct RNA/DNA qPCR Blood (MDX123) on two mammalian RNA targets, in the presence of 5% EDTA blood, were compared using (A) standard qPCR cycling conditions (3 min @ 95ºC, 40 cycles of 10 sec @ 95ºC and 25 sec @ 60ºC), (B) the fastest cycling conditions available on a QuantStudio 7 Flex Real-Time PCR System (3 min @ 95ºC, 40 cycles of 1 sec @ 95ºC and 1 sec @ 60ºC).
Lyophilized format maintains a stable shelf-life for over 18 months
A
B
Lyophilized | Liquid
(A) Lyo-Ready ™ Direct DNA qPCR Blood (MDX122) and (B) Lyo-Ready ™ Direct RNA/DNA qPCR Blood (MDX123) and were lyophilized and the stability of the dried assays ( red ) was tested after 1 month at 37°C against fresh liquid mix ( blue ) in triplex assays in the presence of 5% EDTA blood. The lyophilized mixes retained the same performance as the liquid mix. Results suggest that Lyo-Ready Direct Blood mixes have a projected stability of more than 18 months at ambient temperature.
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