Multiplex testing for COVID-19, Flu and RSV Chinese

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总结

10-15分钟内提供结果,但被认为性能较低,灵敏度较低。 分子和免疫检测,都有各自优缺点,可以单独使用,可以两 者结合使用。通过对流感、COVID和RSV的多重联检,无论采 用哪种检测方法,患者都将能够更快、更有效地得到诊断和 治疗。

同时对多个靶标进行多重检测的方法有几个优点,包括其高通 量潜力、可使用单一样品同时检测多个靶标、以及可以减少试 剂的消耗。这些优势使得多重联检比传统的单重检测有更高的 性价比。 由于许多呼吸道疾病症状相似,仅从临床症状是不可能可靠地 将感染的病原体区分开。在预期高于正常流感季节的情况下, 多重联检能解决在SARS-CoV-2、甲流/乙流和RSV感染之间进 行筛查的更高需求。 分子多重检测具有很高的灵敏度和特异性,可以在疾病早期 得到诊断,并有足够的时间进行适当的治疗。但是分子检测 需要先进的设备,经验丰富的技术人员,通常有1-5天的周转 时间。相比之下,免疫快速抗原测定法使用方便,可以在

参考文献 1 Jong-Yoon Jong. “Multiplex molecular diagnostics: shifting the paradigm.” Medica Laboratory Observer. Feb 17, 2013. Accessed Sept 12, 2023: https://www.mlo-online.com/home/article/13005002/multiplex- molecular-diagnostics-shifting-the-paradigm 2 The University of Sydney. “How COVID-19 created dramatic changes in a ‘winter virus’”. May 31, 2022. Accessed Sept 12, 2023: https://www.sydney.edu.au/news-opinion/news/2022/05/31/how-covid19- created-dramatic-changes--in-a-winter-virus.html 3 Budd, J., Miller, B.S., Weckman, N.E. et al. (2023) Lateral flow test engineering and lessons learned from COVID-19. Nat Rev Bioeng 1, 13–31. https://doi.org/10.1038/s44222-022-00007-3 4 Use of SARS-CoV-2 antigen-detection rapid diagnostic tests for COVID-19 self-testing.4 ), WHO https:// www.who.int/publications-detail-redirect/WHO-2019-nCoV-Ag-RDTs-Self_testing-2022.1 (2022). World Health Organization interim guidance on recommending COVID-19 self-testing using SARS-CoV-2 antigen tests; the web annexes include useful information on implementation 5 World Health Organization. “The ACT-Accelerator: two years of impact”. April 26, 2022. Accessed Sept 12, 2023: https://www.who.int/publications/m/item/the-act-accelerator--two-years-of-impact (2022). 6 Gasperino, D. et al. (2018) Improving Lateral Flow Assay Performance Using Computational Modeling. Annu. Rev. Anal. Chem. 11:219–44. https://doi.org/10.1146/annurev-anchem-061417-125737 7 Centers for Disease Control and Prevention. “Rapid Diagnostic Testing for Influenza: Information for Clinical Laboratory Directors”. https://www.cdc.gov/flu/professionals/diagnosis/rapidlab.htm. Accessed October 26, 2021 8 Centers for Disease Control and Prevention. “RSV in Infants and Young Children”. https://www.cdc.gov/ rsv/high-risk/infants-young-children.html. Accessed October 26, 2021. 9 Meng, J., Stobart, C. C., Hotard, A. L. & Moore, M. L. (2014). An overview of respiratory syncytial virus. PLoS Pathog. 24;10(4):e1004016. doi: 10.1371/journal.ppat.1004016.

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