Air-Dryable Direct Blood

High tolerance to high concentrations of blood (20% K2-EDTA whole blood)

Two viral RNA targets (Human Immunodeficiency Virus (HIV-1) and Hepatitis C virus (HCV)) and one DNA target (Hepatitis B virus (HBV)) were amplified in a triplex reaction using Air-Dryable ™ Direct RNA/DNA qPCR Blood (MDX121) in the presence of 20% K2-EDTA whole blood. The results illustrate the robust and reproducible performance achieved with Air-Dryable ™ Direct RNA/DNA qPCR Blood in the presence of high concentrations of whole blood.

HCV

HBV

HIV

High tolerance to whole blood stabilized with anticoagulants (K2-EDTA, sodium heparin, and sodium citrate)

Anticoagulants and high concentrations of whole blood are known to inhibit qPCR efficiencies. 0% 2%, 5% and 20% human whole blood in the presence of K2-EDTA ( orange ), sodium heparin ( green ) and sodium citrate ( grey ) was tested with (A) Air-Dryable Direct DNA qPCR Blood (MDX092) against mixes from KAPA Probe Force (Roche) and TaqPath ™ (Thermo) and (B) Air-Dryable Direct RNA/DNA qPCR Blood (MDX121) against mixes from Ultraplex ™ (QuantBio) and TaqPath ™ 1-Step (Thermo). The results demonstrate that the reaction efficiencies of the Air-Dryable Direct Blood mixes are higher both in the presence of anticoagulants and high concentrations of blood than of other suppliers’ mixes.

A) Air-Dryable ™ Direct DNA qPCR Blood (MDX092)

B) Air-Dryable ™ Direct RNA/DNA qPCR Blood (MDX121)

MDX092

KAPA Probe Force (Roche)

TaqPath ™ (Thermo)

MDX121

Ultraplex ™ (QuantBio)

TaqPath™ 1-Step (Thermo)

K2-EDTA | Sodium heparin | Sodium citrate

Air-drying does not impact assay efficiency or sensitivity Air-Dryable ™ Direct DNA qPCR Blood (MDX092)

20% K2-EDTA whole blood

20% serum

Air-dried | Liquid | KAPA Probe Force (Roche)

Activity of Air-Dryable Direct DNA qPCR Blood (MDX092) in both air-dried ( red ) and liquid ( blue ) formats were compared to a liquid mix from KAPA Probe Force (Roche, green ) in a multiplexing qPCR assay, using a 10-fold serial dilution of plasmid DNA (10,000, 1000, 100 and 10 copies respectively) for Plasmodium falciparum , in the presence of (A) 20% K2-EDTA whole blood and (B) 20% K2-EDTA serum. The results illustrate that the air-dried Air-Dryable Direct DNA qPCR Blood retains the ability to efficiently amplify to the same level as the liquid mix and shows higher end florescence and sensitivity than the KAPA Probe Force (Roche).