Molecular Reagents
Optimized RT-qPCR Mixes
Meridian’s portfolio of RT-qPCR master mixes have been designed to simplify the development of SARS-CoV-2 molecular assays and enable new features such as extraction-free amplification. Each master mix contains a hot-start polymerase, dNTPs, buffer and other components optimized for each particular application (e.g. lyophilization). Only primers and probes are required to complete the assay formula. SARS-CoV-2 requires an RNA extraction step which is time limiting and can result in RNA being lost during the extraction process. However, extraction is typically required to remove inhibitors which would otherwise impact an assay’s sensitivity and accuracy. In order to address this challenge, Meridian has developed several inhibitor-tolerant mixes capable of delivering sensitive multiplex detection, even in the presence of difficult inhibitors found in sputum, saliva and stool specimens. This new mix offers a novel alternative to nucleic acids extraction, saving both time and labor which are vitally important in viral screening assays that require rapid detection for infection control.
DNA/RNA purification Add to master mix
2
3
Sample
Go to PCR
1
4
Conventional approach
Meridian’s Direct Solution
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3
Sample
Go to PCR
No DNA/RNA extraction needed
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Specimen-Specific Sample Mix
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