RT-qPCR Master Mixes
Inhibitor-Tolerant RT-PCR Mix
PRODUCT HIGHLIGHTS
PRODUCT
CAT NO. VOLUME REACTIONS
• Designed for amplification from crude samples such as blood, urine, cerebral spinal fluid (CSF) and milk • 4x concentrated - ideal for developing qualitative assays where sample is limited • Sensitive amplification in singleplex and multiplex assays
5 mL
500 Rxns
Inhibitor-Tolerant qPCR Mix
MDX013
100 mL 10,000 Rxns
5 mL
100 Rxns
Inhibitor-Tolerant RT-qPCR Mix
MDX016
100 mL 10,000 Rxns
Exhibits a high tolerance to PCR inhibitors from clinical samples
Sputum Specimens Amplification profile of inactivated influenza virus spiked into samples containing 5% sputum or no sputum. The data illustrates that the performance of Inhibitor-Tolerant RT-qPCR Mix (MDX016) in the presence of 5% artificial sputum ( red ) is the same Fast One-Step RT-qPCR Mix (MDX032) with no sputum ( blue ). In contrast, the sensitivity and performance of Fast One-Step RT-qPCR Mix (MDX032) significantly decreases in the presence of 5% artificial sputum ( black ) compared to no sputum or compared to Inhibitor-Tolerant RT-qPCR Mix (MDX016) with sputum.
MDX016 (Artificial sputum) | MDX032 (No sputum) | MDX032 (Artificial sputum)
Saliva Specimens MDX016 ( red ) and MDX032 ( black ) amplification traces of Influenza A spike in presence of saliva swabs (COPAN ESwab 359C) at 10% (left) and 20% (right) final concentration. With earlier Ct (approx. 4 Ct) and higher fluorescence (approx. +50%), the results demonstrate the superiority of Inhibitor-Tolerant RT-qPCR Mix (MDX016) against a standard RT-qPCR Mix (MDX032) for the detection of viral RNA in presence of saliva swab resuspension in UTM.
10% Saliva
20% Saliva
MDX016 | MDX032
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