RT-qPCR Master Mixes
Air-Dryable TM Direct RNA/DNA qPCR Saliva
PRODUCT HIGHLIGHTS
Sensitive detection in multiplex assays using saliva samples (up to 60% human saliva) or Universal Transport Media (up to 35% UTM) Three respiratory pathogens, Influenza A, Middle East Respiratory syndrome coronavirus (MERS-CoV) and Respiratory Syncytial Virus (RSV) were amplified in a triplex qPCR assay in the presence of 35% Universal Transport Media (UTM) with artificial sputum swab. The results illustrate that a higher performance was achieved with Air-Dryable ™ Direct RNA/DNA qPCR Saliva ( red ) compared to TaqPath RT-qPCR Mix ( black ) and UltraPlex 1-Step Tough Mix ( grey ). PRODUCT • Specimen-specific mix designed for singleplex or multiplex assays detecting RNA or DNA pathogens at very low titers from saliva or sputum samples • Ideal for POC diagnostic platforms or automated high-throughput instruments • Can be used in a wet or dry format, and compatible with a range of air-drying protocols to produce an ambient temperature stable mix CAT NO. VOLUME REACTIONS Air-Dryable ™ Direct RNA/DNA qPCR Saliva MDX131 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Influenza A
MERS-CoV
RSV
Air-dried MDX131 | TaqPath RT-qPCR | UltraPlex 1-Step Tough Mix
Lyo-Ready TM Direct RNA/DNA qPCR Saliva
PRODUCT HIGHLIGHTS
• Specimen-specific mix designed for singleplex or multiplex assays detecting RNA or DNA pathogens at very low titers from saliva or sputum samples • Suitable for direct amplification from crude clinical specimens • Can be used in a wet or dry format and is compatible with a range of lyophilization protocols
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL
1,000 Rxns
Lyo-Ready ™ Direct DNA qPCR Saliva
MDX132
50 mL
10,000 Rxns
5 mL
1,000 Rxns
Lyo-Ready ™ Direct RNA/DNA qPCR Saliva
MDX133
50 mL
10,000 Rxns
Sensitive detection from sputum samples containing Universal Transport Media (up to 50% UTM) Three respiratory pathogens, Influenza A, Middle East Respiratory syndrome coronavirus (MERS-CoV) and Respiratory Syncytial Virus (RSV) were amplified in a triplex qPCR assay in the presence of 50% Universal Transport Media (UTM) swab containing sputum. The results illustrate that a higher multiplexing capacity and reproducibility was achieved with lyophilized Lyo-Ready ™ Direct RNA/DNA qPCR Saliva ( red ) compared to TaqPath ™ 1-Step Multiplex Master Mix ( black ) or UltraPlex ® 1-Step ToughMix ( grey ).
Influenza A
MERS-CoV
RSV
Air-dried MDX133 | TaqPath RT-qPCR | UltraPlex 1-Step Tough Mix
MeridianLifeScience .com
Powered by FlippingBook