Inhibitor-Tolerant RT-qPCR Mix
• Designed for amplification from crude samples without the need for complex or time-consuming extraction • 4x concentration master mix allows for greater sample volume • Single tube, one-step mix. Only add primers, probes and clinical samples • Lyo-ready master mix can be lyophilized, with primers and probe to create an ambient temperature stable assay • Suitable for single and multiplex detection of RNA and DNA pathogens
PRODUCT
CAT NO.
VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 1,000 Rxns 50 mL 10,000 Rxns 5 mL 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Inhibitor-Tolerant RT-qPCR Mix, 4x
MDX016
Inhibitor-Tolerant RT-qPCR Low-ROX Mix, 4x Lyo-Ready Inhibitor-Tolerant RT-qPCR Mix, 4x
MDX105
MDX185
Inhibitor-Tolerant RT-qPCR Mix exhibits a high tolerance to PCR inhibitors from clinical samples
SALIVA SPECIMENS
10% Saliva
20% Saliva
MDX016 (red) and a standard RT-qPCR Mix (black) amplification traces of Influenza A spiked into saliva swab matrix (COPAN ESwab 359C), used at final concentrations of 10% (left) and 20% (right). With earlier Ct (approx. 4 Ct) and higher fluorescence (approx. +50%), the results demonstrate the superiority of Inhibitor-Tolerant RT-qPCR Mix (MDX016) against a standard RT-qPCR Mix for the detection of viral RNA in presence of saliva swab resuspension in UTM.
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