Air-dryable mix demonstrates high sensitivity of pathogens across a wide dynamic range (10 6 to 10 cfu) in a wet or dry format
A) Staphylococcus aureus amplification plot
B) Listeria monocytogenes amplification plot
Staphylococcus aureus standard curve
Listeria monocytogenes standard curve
Figure 1 . Using Air-Dryable ™ qPCR Mix (MDX082), amplification of 16s genes from, A/ Staphylococcus aureus and B/ Listeria monocytogenes was compared in a 10-fold dilution series before ( blue ) and after ( red ) air-drying. The results illustrated that as low as 10 cfu/rxn could be detected and that the reaction efficiencies were also maintained before and after drying at 98% and 100% respectively.
Air-dryable mix exhibits high inhibitor tolerance and provides sensitive results using crude samples
Figure 2 . Amplification of 10-fold serial dilution of Hepatitis A RNA using air-dried Air-Dryable ™ 1-Step RT-qPCR Mix (MDX095) in presence of 0.8% seafood extract. The results
illustrated the sensitivity of the Air-Dryable ™ 1-Step RT-qPCR Mix, being able to detect very low levels of Hepatitis A, even in a crude sample.
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