Benefits to Room-Temperature Stable Assays Room-temperature stable assays are becoming increasingly popular with test manufacturers looking to simplify assay handling and processing. The main benefits are eliminating the need for cold storage, increasing the shelf life of products at ambient temperatures and enabling ‘one tube’ assay protocols. Overall, ambient-temperature stable assays help reduce the cost of shipping and storage and increase the life of the product. Air-Dryable RT-qPCR and qPCR mixes are easy to use and ideal for developing ambient temperature, multiplex molecular assays. They are glycerol-free and contain Taq polymerase, reaction buffer, dNTPs, MgCl 2 and air-dry compatible excipients. In order to produce room temperature air-dried qPCR reagents, assay specific primers and probes can be added to Air-Dryable qPCR Mix for subsequent air-drying.
Stable shelf-life for up to 12 months
Figure 5 . Air-Dryable ™ qPCR Mix was air-dried, and the stability was tested in an accelerated stability study. The air-dried mix was incubated at 37°C for 8 weeks ( black ) and tested against a freshly prepared mix ( red ) by qPCR assay on 100-fold template dilutions. Results suggest that the air-dried mix is active following accelerated stability tests with projected 12 months stability at ambient temperature.
Reference | 37°C
Optimized Master Mixes
MDX030 Low DNA qPCR Mix Designed for multiplex assays detecting microbial or fungal DNA. Incorporates a heat-activated DNA polymerase with low residual DNA content. MDX020 Fast qPCR Mix Ideal for multiplex assays requiring sensitive detection of DNA targets in inhibitor-rich samples. Contains an antibody-mediated hot-start polymerase. MDX032 Fast 1-Step RT-qPCR Mix Ideal for multiplex assays requiring sensitive detection of DNA and RNA targets. Contains an antibody-mediated hot-start polymerase, MMLV-RT and separate RNase inhibitor. MDX013 Inhibitor-Tolerant qPCR Mix Designed for amplification direct from crude lysates or inhibitor-rich samples such as urine, cerebral spinal fluid (CSF), blood as well as plants. Contains an antibody-mediated hot-start polymerase. MDX016 Inhibitor-Tolerant RT-qPCR Mix All-in-one, 4x master mix designed for RT-qPCR from crude samples such as sputum, stool and saliva. No sample extraction required.
MDX082 Air-Dryable ™ qPCR Mix, 4x Ready-to-use qPCR mix ideal for oven or air-drying technologies. MDX095 Air-Dryable ™ RT-qPCR Mix, 4x Ready-to-use RT-qPCR Mix ideal for oven or air-drying technologies. MDX021 Lyo-Ready ™ qPCR Mix Ready-to-use, glycerol-free qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets. MDX024 Lyo-Ready ™ 1-Step RT-qPCR Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophiliization into beads or pellets. MDX062 Lyo-Ready ™ 1-Step RT-qPCR Virus Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients and highly suited for amplification of RNA viruses with a high secondary structure. MDX097 Lyo-Ready ™ LAMP Mix Ready-to-use, glycerol-free RT-qPCR master mix formulated with a specialized blend of excipients for lyophilization into beads or pellets.
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