STEP 6 LIBRARY AMPLIFICATION
Glycerol-Free High-Fidelity Pfu (HC) (MDX203) is a high-fidelity, thermostable DNA-dependent DNA polymerase from Pyrococcus furiosus . The 3’ to 5’ proofreading exonuclease activity of Pfu polymerase makes it suitable for applications that require high-fidelity DNA synthesis or blunt-ended PCR fragments. Glycerol-Free High-Fidelity Pfu (HC) is supplied in a glycerol-free storage buffer and is accompanied by a 5x Reaction Buffer that contains magnesium, dNTPs and excipients required for lyophilization. Glycerol-Free High-Fidelity Pfu (HC)
Cat #MDX203 VOLUME
UNITS
Product Highlights • High concentration Pfu
100 μ L 500 10 mL 20,000
polymerase allowing for bulk production of PCR master mixes. • Ideal for NGS library amplification. • Glycerol-free, ideal for preparation of dried-down PCR tests • Concentration 20 U/ µ L
Maintains multiplexing performance after lyophilization
(Left) Glycerol-Free High-Fidelity Pfu (HC) (MDX203), stored at 37°C for 1 and 4 weeks. Lyophilized samples were tested against the same enzyme and reaction buffer without lyophilisation (Wet Reference) in multiplexed PCR amplification of 7 targets of various GC content: 793 bp (32% GC), 649 bp (54% GC), 548 bp (60% GC) , 418 bp (48% GC), 332 bp (48% GC), 196 bp (51% GC), 135 bp (55% GC). Results show that the lyophilized MDX203 is stable with no loss of activity for at least 1 month at 37°C with projected ambient temperature stability of 1 year.
Wet Reference
Lyo Day 7 at 37°C
Lyo Day 28 at 37°C
Lyo Day 0
6
Powered by FlippingBook