Next-Generation Sequencing (NGS)

ADDITIONAL NGS PRODUCTS

Meridian’s NGS Clean and Select Beads (MDX041) are paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries. Paramagnetic beads selectively bind DNA fragments based on the volume ratio of bead suspension and sample. They are delivering highly purified DNA fragments, efficiently removing contaminants, such as nucleotides, primers, adapters, enzymes, buffer additives and salts. NGS Clean and Select Beads

Cat #MDX041

VOLUME 50 mL 500 mL

Product Highlights • Paramagnetic beads for

clean-up and size selection. • Tuneable size selection protocols for single or double-sided selection. • C ompatible with manual and automated workflows.

Left-side and right-side size selection of DNA fragments

(Left) For double-sided size selection, supernatant from a right-side selection step (which excludes the largest library fragments) is re-purified using a different ratio of beads, in a left-side purification step to exclude the smallest library fragments.

Library Quantification Kit

Cat #MDX039

VOLUME REACTIONS 5 mL 500

Meridian’s Library Quantification Kit (MDX039) is a qPCR-based assay for the exclusive quantification of adapter-ligated molecules providing accurate quantification of Illumina NGS libraries. It contains pre-diluted standards to minimize pipetting errors, a P5 / P7 Illumina ® specific primer mix and an optimized buffer for dilution of NGS library samples.

Product Highlights • Suitable for quantification of all Illumina compatible libraries. • Accurate results in less than 90 minutes. • C ontains six pre-diluted DNA standards for rapid and reliable standard curve generation.

qPCR-based Quantification of NGS Libraries

(Left) Standard curve generated from the amplifications of the 6 pre-diluted DNA standards ( blue , 10 pM to 100 aM) and 3 dilutions of an Illumina ® NGS library ( red , ten-fold series).

6 pre-diluted DNA standards | Illumina ®