ADDITIONAL NGS PRODUCTS
Meridian’s NGS Clean and Select Beads (MDX041) are paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries. Paramagnetic beads selectively bind DNA fragments based on the volume ratio of bead suspension and sample. They are delivering highly purified DNA fragments, efficiently removing contaminants, such as nucleotides, primers, adapters, enzymes, buffer additives and salts. NGS Clean and Select Beads
Cat. # MDX041
VOLUME 50 mL 500 mL
Product Highlights • Paramagnetic beads for clean-up and size selection • Tuneable size selection protocols for single- or double-sided selection • Compatible with manual and automated workflows
Left-Side and Right-Side Size Selection of DNA Fragments
For double-sided size selection, supernatant from a right-side selection step (which excludes the largest library fragments) is re-purified using a different ratio of beads in a left-side purification step to exclude the smallest library fragments.
Meridian’s NGS Library Quantification Kit (MDX039) is a qPCR-based assay for the exclusive quantification of adapter-ligated molecules, providing accurate quantification of Illumina ® NGS libraries. It contains pre-diluted standards to minimize pipetting errors, a P5 / P7 Illumina ® -specific primer mix and an optimized buffer for dilution of NGS library samples. NGS Library Quantification Kit
Cat. # MDX039
VOLUME REACTIONS 5 mL 500
Product Highlights • Suitable for quantification of all Illumina ® - compatible libraries • Accurate results in less than 90 minutes • Contains six pre-diluted DNA standards for rapid and reliable standard curve generation
qPCR-Based Quantification of NGS Libraries
6 pre-diluted DNA standards | Illumina ® ®
Standard curve generated from the amplifications of the 6 pre-diluted DNA standards ( blue , 10 pM to 100 aM) and 3 dilutions of an Illumina ® NGS library ( red , ten-fold series).
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