APPLICATION NOTE qPCR MIXES FOR tNGS
To support amplicon-based tNGS workflows, Meridian provides a modular portfolio of high-performance, glycerol-free reagents—including optimized mixes, enzymes and nucleotides—engineered for sensitive amplification, efficient clean-up and seamless integration into your workflow. From PCR through library preparation , our solutions are designed to reduce hands-on time, improve assay consistency and deliver reliable results at scale. FROM PCR TO LIBRARY PREP REAGENTS ENGINEERED FOR RELIABILITY
Here’s How Meridian Fits into Your tNGS Workflow:
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REFERENCES 1. Zheng, Y. R., et al. (2024). Comparison of targeted next-generation sequencing and metagenomic next-generation sequencing in the identification of pathogens in pneumonia after congenital heart surgery: a comparative diagnostic accuracy study. Italian Journal of Pediatrics , 50 (1), 174. https://doi.org/10.1186/s13052-024-01749-z 2. Broude, N. E., et al . (2001). Multiplex allele-specific target amplification based on PCR suppression. Proc Natl Acad Sci USA , 98 (1), 206-211. https://doi.org/10.1073/pnas.98.1.206 3. Merino, D. M., et al. (2020). Establishing guidelines to harmonize tumor mutational burden (TMB): In silico assessment of variation in TMB quantification across diagnostic platforms: phase I of the Friends of Cancer Research TMB Harmonization Project. Journal for ImmunoTherapy of Cancer, 8, e000147. https://doi.org/10.1136/ jitc-2019-000147 4. Allied Market Research, Next Generation Sequencing Market Forecast, 2023-2035, April 2024.
Discover how Meridian’s molecular solutions can help optimize your targeted NGS workflows. From high-performance PCR chemistries to custom formulations and scalable manufacturing, we provide the tools and expertise to accelerate your assay development. VISIT: meridianbioscience.com/lifescience/ precision-oncology-solutions
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