Superior sensitivity with shorter time-to-results (TTR) A) Air-Dryable ™ DNA LAMP (MDX119)
B) Air-Dryable ™ RNA/DNA LAMP (MDX118)
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1000 copies/rxn 100 copies/rxn
20 copies/rxn
NTC
1000 copies/rxn 500 copies/rxn 100 copies/rxn
10 copies/rxn
The average time to results (TTR) of Air-Dryable ™ LAMP Mixes in both air-dried (red) and liquid (blue) formats vs. NEB WarmStart ® (orange). Results demonstrate greater sensitivity of Air-Dryable ™ LAMP Mixes with shorter TTR across all template concentrations. (A) Conserved regions from the S and P genes of Hepatitis B Virus (1000, 100 and 20 copies respectively) were amplified using Air-Dryable ™ DNA LAMP (MDX119) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart ® ( orange ). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. At lower copy numbers, NEB WarmStart ® ( orange ) exhibited significantly lower reaction reproducibility compared to Air-Dryable ™ DNA LAMP Mix, and a lower TTR was observed for reactions using Air-Dryable ™ DNA LAMP Mix across all dilutions of the Hepatitis B gene. (B) Emesvirus (MS2) was amplified using Air-Dryable ™ RNA/DNA LAMP (MDX118) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart ® ( orange ). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Error bars represent standard deviation over 4 technical replicates. A significantly lower TTR was observed for reactions using Air-Dryable ™ DNA/RNA LAMP Mix across all dilutions of MS2-RNA.
High reaction specificity with little-to-no non-specific amplification
Air-Dryable ™ RNA/DNA LAMP (MDX118) ( blue ) was compared to NEB WarmStart ® ( orange ) for detection of EBV DNA (1000 ipc) and MS2 RNA (1000 ipc). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Amplification plots of 3 technical replicates show Air-Dryable ™ RNA/DNA LAMP mix has earlier TTR and no non-specific amplification compared to NEB WarmStart ® which has detectable amplification for non-template controls (NTC).
EBV-DNA
MS2-RNA
Shelf-life greater than 12 months after air-drying A) Air-Dryable ™ DNA LAMP
B) Air-Dryable ™ RNA/DNA LAMP
Air-Dryable ™ DNA LAMP and RNA/DNA LAMP mixes were air-dried and their stability was tested in an accelerated stability study. A) 9TV10 from Trichomonas vaginalis was amplified with Air-Dryable ™ DNA LAMP and B) Dengue Type 2 was amplified with Air-Dryable ™ RNA/DNA LAMP that was air-dried ( red ) and incubated a 37°C for over 1 month and tested against the fresh liquid mix ( blue ). Results suggest that the air-dried mixes retain full activity following accelerated stability tests with a projected stability of greater than 12 months at ambient temperature.
20 16 12
20 16 12
8 4 0
8 4 0
Time 0
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2 weeks 2 weeks
Time 0 Liquid
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3 weeks
5 weeks
Air-Dried
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