ANTIBODIES & ANTIGEN CONTROLS USED IN STOOL ANTIGEN DETECTION ASSAYS
Gastrointestinal Pathogens for Stool Testing ANTIBODIES & ANTIGEN CONTROLS USED IN STOOL ANTIGEN DETECTION ASSAYS
Meridian offers a range of antibodies and antigens for use in various immunoassays to detect gastrointestinal pathogens. These products are currently being used in validated, commercial diagnostic assays to detect pathogens from stool samples
COMMON GASTRO PATHOGENS • Adenovirus • Astrovirus • Calprotectin
• Enterovirus • Giardia lamblia • Helicobacter pylori • Lactoferrin • Listeria monocytogenes • Norovirus • Rotavirus • Salmonella • Shigella • Verotoxin • Yersinia enterocolitica
• Campylobacter coli • Campylobacter jejuni • Clostridium Botulinum • Clostridium difficile • Cryptosporidium parvum • E. coli O157 • Entamoeba species
DIAGNOSTICS FOR ACUTE GASTROENTERITIS
Acute gastroenteritis is a common syndrome that is generally caused by a virus, parasite or food-borne bacteria. Diagnosis of the responsible intestinal pathogen requires the detection of the organism in the feces, duodenal fluid, or small intestine using biopsy specimens. Since fecal examination is labor intensive and requires a skilled microscopist, antigen detection tests have been developed as alternatives using immunofluorescent assays (IFA), enzyme immunoassay (EIA), and rapid diagnostic tests (RDTs) in various formats. Some of these immunoassays are quick to perform, do not require skilled personnel and are very specific. There are many commercially available diagnostics for a range of gastrointestinal pathogens, including panel assays that simultaneously test for a range of possible infectious agents using a single stool sample.
Large panel of matched antibody pairs Antigens available for use as controls
Pairs demonstrated to work in ELISA, LF or both All suitable for development in stool based assays
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ANTIBODIES FOR STOOL TESTING
Adenovirus Hexon C11994M
Clostridium difficile Toxin A BN1103 ELISA, LF, pair BN1104 ELISA, LF, pair BN1105 ELISA, LF, pair C70517M ELISA C01677M ELISA, pair C01678M ELISA, pair Clostridium difficile Toxin B BN1106 ELISA, LF, pair BN1107 ELISA, LF, pair C65427M ELISA C65423M ELISA, LF, pair C65426M ELISA, LF, pair Cryptosporidium parvum C65370M ELISA, IFA, IHC C01948M LF, pair E. coli O157 C65160M ELISA, IFA C01533M ELISA, IFA, pair C01535M ELISA, IFA, pair C01958M LF, pair Entamoeba histolytica & dispar C01945M LF, pair Giardia lamblia C01597M ELISA, IFA, WB C01950M (trophozoite) LF, pair C01949M (trophozoite) LF, pair C65361M ELISA, IFA, WB, pair C65612M ELISA, IFA, WB, pair Helicobacter pylori C65461M ELISA, IFA, WB C01956M LF, pair Lactoferrin H01412M LF, pair H01411M LF, pair Norovirus GI Capsid C01871M ELISA, LF, WB, pair C01872M ELISA, LF, WB, pair C01875M ELISA, WB, pair
Norovirus GII Capsid BN1047
ELISA, LF
ELISA, LF, pair ELISA, LF, pair
C01727M C01728M C01554M C01256M C65431M C65604M
ELISA, LF, pair ELISA, LF, pair
BN1048
C01877M C01878M C01879M C01880M
ELISA, LF, WB, pair
ELISA, LF, IFA, pair ELISA, LF, IFA, pair ELISA, LF, IFA, pair ELISA, LF, IFA, pair
ELISA, pair
ELISA, LF, WB, pair ELISA, LF, WB, pair
Rotavirus C65194M
C01953M LF, pair Adenovirus 40 & 41 C66431M ELISA Astrovirus C01981M
ELISA, LF, pair ELISA, LF, pair ELISA, LF, pair ELISA, LF, pair ELISA, LF, pair
C65196M C65197M C01729M C01730M
ELISA, IFA, IHC, pair ELISA, IFA, IHC, pair ELISA, IFA, IHC, pair
Rotavirus Group A Specific Antigen, VP6 C66130M ELISA Salmonella C01918M
C01982M C01983M C01984M C01955M
ELISA, pair
LF, pair
C01954M LF, pair C. coli and C. jejuni C01946M LF, pair C01947M LF, pair Calprotectin L1 K01375M ELISA K01373M K01376M ELISA, pair Campylobacter jejuni C66700M ELISA C65727M ELISA, pair C. botulinum Toxin E BN1157
ELISA, IFA, LF, pair
Salmonella species (A,B,C,D,E,F & G Groups) C65957M ELISA, IFA C01360M ELISA, IFA Salmonella paratyphi A C01884M ELISA, IFA C01921M ELISA, IFA, pair Salmonella typhi (LPS) BN1150 ELISA, LF BN1151 ELISA, LF C01362M ELISA, IFA, LF, pair C01919M ELISA, IFA, LF, pair Shigella B65901R ELISA, IFA Verotoxin I (Shiga-Like) B Subunit (SLT-1b, STX-1b) C01690M ELISA Verotoxin II (Shiga-like) A subunit (SLT-2a, STX-2a) C65585M ELISA, Neutralizing
ELISA, LF, WB, pair
ELISA, LF, pair ELISA, LF, pair ELISA, LF, pair
BN1158 BN1159
BN1160 ELISA, LF, pair Clostridium difficile GDH BN1108 ELISA, LF, pair BN1109 ELISA, LF, pair C01957M LF, pair
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ANTIGENS FOR STOOL TESTING
Several antigens have been tested with antibody pairs. See page 4 for further information.
Clostridium difficile GDH BN1143 LF BN1144 LF R01738, E. coli LF, pair Cryptosporidium parvum A01709N, Native LF, pair E. coli 0157 A01718N, Native LF R01741, E. coli LF R01742, E. coli LF
Astrovirus R01729, E. coli Calprotectin R01724, E. coli
Listeria monocytogenes A01710N LF Norovirus GI BN1135 LF R01731, E. coli LF R01730, E. coli LF R01744, Insect LF Norovirus GII BN1133 LF BN1134 LF R01732, E. coli LF R01733, E. coli LF R01734, E. coli LF R01745, E. coli LF Rotavirus R01728, E. coli LF Salmonella A01699N, Native (typhi) , LF A01716N, Native (typhi) , LF A01700N, Native ( paratyphi A), LF A01701N, Native ( paratyphi B), LF A01702N, Native ( enteritidis ), LF BN1146 LF Shigella A01704N, Native (boydii) , LF A01705N, Native (dysenteriae) , LF A01706N, Native (sonnei) , LF A01721N, Native (flexneri) , LF Yersinia enterocolitica
LF, pair
LF Campylobacter coli A01713N, Native LF, pair R01720, E. coli LF, pair
Campylobacter jejuni A01714N, Native LF, pair R01721, E. coli LF, pair Clostridium difficile Toxin A R01739, E. coli LF Clostridium difficile Toxin B BN1145 LF R01740, E. coli LF
Entamoeba R01718, E. coli R01719, E. coli Enterovirus R01735, E. coli R01736, E. coli R01737, E. coli R01763, E. coli R01764, E. coli R01765, E. coli
(histolytica) , LF, pair
(dispar) , LF, pair
LF LF LF LF LF
LF Giardia lamblia R01723, Yeast LF R01722, E. coli LF, pair Helicobacter pylori A01722, Native LF, pair Lactoferrin A01712H, Native LF, pair
A01707N, Native LF A01708N, Native LF
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Antibody Pairs
CAPTURE C01727M C65431M C01953M C65431M C65431M C01955M C01981M C01981M C01984M C01984M K01373M
DETECTION
ANTIGEN
APPS
C01728M C01554M C01953M C01256M C65604M C01954M C01982M C01983M C01982M C01983M K01376M
ELISA & LF ELISA & LF
Adenovirus
LF
ELISA & LF ELISA & LF
R01729
LF
ELISA & IFA ELISA & IFA
Astrovirus
ELISA ELISA ELISA
Calprotectin L1
BN1160 BN1160 BN1160
BN1158 BN1157 BN1159
ELISA & LF ELISA & LF ELISA & LF
C. botulinum Toxin E:
A01714N ( C. jejuni ) (Native) A01713N ( C. coli ) (Native)
LF LF LF LF
C. coli and C. jejuni
C01947M
C01946M
R01721 ( C. jejuni OMP) (Recombinant) R01720 ( C. coli OMP) (Recombinant)
C01678M
C01677M
ELISA
C. difficile Toxin A
BN1104 BN1104
BN1105 BN1103
LF LF LF LF LF LF
C65423M
C65426M
C. difficile Toxin B
BN1107
BN1106
C01957M
C01957M
R01738
C. difficile GDH
BN1108
BN1109
C. jejuni
C65727M C01948M C01535M C01958M
C65727M C01948M C01533M C01958M
ELISA & LF
C. parvum
A01709N (Native)
LF
ELISA
E. coli O157
LF LF LF LF
R01718 (E. histolytica) (Native) R01719 (E. dispar) (Native)
Entamoeba
C01945M
C01945M
C01950M C65612M C01956M H01412M
C01949M C65361M C01956M H01411M
R01722 (Recombinant)
G. lamblia
ELISA
H. pylori
A01722N
LF LF
Lactoferrin Leptospira
A01712H (Native)
BN1153
BN1152
ELISA & LF
C01872M C01873M C01871M C01874M C01880M C01880M C01879M C01877M C01877M
C01871M C01875M C01875M C01875M C01878M C01880M C01877M C01878M C01877M
LF
ELISA & WB
Norovirus GI Capsid
ELISA ELISA
LF LF LF
Norovirus GII Capsid
ELISA ELISA
BN1048
BN1047
ELISA & LF ELISA & LF ELISA & LF ELISA & LF ELISA & LF
C65196M C65196M C01729M
C65194M C65197M C01730M
Rotavirus
BN1150
BN1151
C01919M C01921M C01362M
C01918M C01918M C01362M
ELISA, IFA & LF
Salmonella
ELISA & IFA
ELISA, IFA & LF
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
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