Respiratory Diseases

INFLUENZA B - Reagents for serology testing Antigen Detection Assays Influenza | Continued

R02310

Influenza B Antigen (B/Hong Kong 5/27) • Source: MDCK cells • Purified via concentrated cell culture supernatant • Concentration: 0.72 mg/mL (Dye Binding Assay) /HA Titer: 45,511 HA Units/mL • Buffer: EMEM, pH 7.0 • Inactivation: Gamma radiation Influenza B Antigen (B/Malaysia) • Source: Chicken eggs • Purified: > 90% pure (SDS-PAGE). Ultracentrifugation with 10-40% sucrose gradient • Concentration: 2.5mg/mL • Buffer: 0.05 M Tris-HCl, pH 8.0 containing 0.1 M Sodium Chloride, 5 mM EDTA • Inactivation: Thimerosal and beta propiolactone treatment

ELISA, Control Antigens

R01248

INFLUENZA B - Reactivity data Current circulating influenza B strains are changing profile. The World Health Organization (WHO) analysis of circulating influenza B strains has revealed that while Victoria lineage viruses are prevalent in some countries, the proportion of Yamagata lineage ones continue to increase and are becoming dominant in many countries. Patterns with the genetic clades showed that many viruses in clade 2, which includes B/Massachusetts/2/2012, were antigenically distinct from those in clade 3, which includes B/Wisconsin/1/2010. As a result WHO recommends including B/Massachusetts/2/2012, in replacement of B/Wisconsin/1/2010, and a B/Brisbane/60/2008-like virus starting from the 2013-14 season. The following antibodies have been tested against a panel of influenza subtype nucleoproteins using a immunoprecipitation-equivalent method to determine their reactivity to a particular strain. The higher the percentage, the stronger the reactivity of the antibody.

Inactivated Virus

Unknown Lineage

Unknown Lineage B/Tokio/ 53/99

Victoria-Lineage

Yamagata-Lineage

Cat No.

B/Malaysia/ 2506/2004

B/Brisbane/ 60/2008

B/Qingdao/ 102/91

B/Victoria/ 504/00

B/Wisconsin/ 01/2010

B/Massachusetts /2/2012

90

88

70

71

71

88

90

C01741M

89

90

98

96

97

90

91

C01742M

96

95

98

97

99

97

95

C01743M

86

85

95

95

98

93

91

C01744M

88

87

96

96

99

91

90

C01745M

88

87

95

92

98

90

89

C01746M

91

94

94

84

98

94

97

C01747M

96

95

99

99

100

96

97

C01748M

95

95

75

78

82

95

95

C01749M

20

Respiratory Diseases - Reagents for Assay Development

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