Influenza B Antigen (B/Hong Kong 5/27) • Source: MDCK cells • Purified via concentrated cell culture supernatant • Concentration: 0.72 mg/mL (Dye Binding Assay) /HA Titer: 45,511 HA Units/mL • Buffer: EMEM, pH 7.0 • Inactivation: Gamma radiation Influenza B Antigen (B/Malaysia) • Source: Chicken eggs • Purified: > 90% pure (SDS-PAGE). Ultracentrifugation with 10-40% sucrose gradient • Concentration: 2.5mg/mL • Buffer: 0.05 M Tris-HCl, pH 8.0 containing 0.1 M Sodium Chloride, 5 mM EDTA • Inactivation: Thimerosal and beta propiolactone treatment
R02310
ELISA, Control Antigens
R01248
INFLUENZA B - Reactivity data Current circulating influenza B strains are changing profile. The World Health Organization (WHO) analysis of circulating influenza B strains has revealed that while Victoria lineage viruses are prevalent in some countries, the proportion of Yamagata lineage ones continue to increase and are becoming dominant in many countries. Patterns with the genetic clades showed that many viruses in clade 2, which includes B/Massachusetts/2/2012, were antigenically distinct from those in clade 3, which includes B/Wisconsin/1/2010. As a result WHO recommends including B/Massachusetts/2/2012, in replacement of B/Wisconsin/1/2010, and a B/Brisbane/60/2008-like virus starting from the 2013-14 season. The following antibodies have been tested against a panel of influenza subtype nucleoproteins using a immunoprecipitation-equivalent method to determine their reactivity to a particular strain. The higher the percentage, the stronger the reactivity of the antibody.
Inactivated Virus
Unknown Lineage
Unknown Lineage B/Tokio/ 53/99
Yamagata-Lineage
Victoria-Lineage
Cat No.
B/Malaysia/ 2506/2004
B/Brisbane/ 60/2008
B/Qingdao/ 102/91
B/Victoria/ 504/00
B/Wisconsin/ 01/2010
B/Massachusetts /2/2012
C01741M C01742M C01743M C01744M C01745M C01746M C01747M C01748M C01749M
90 89 96 86 88 88 91 96 95
88 90 95 85 87 87 94 95 95
70 98 98 95 96 95 94 99 75
71 96 97 95 96 92 84 99 78
71 97 99 98 99 98 98
88 90 97 93 91 90 94 96 95
90 91 95 91 90 89 97 97 95
100
82
27
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