High-Sensitivity SARS-CoV-2 Nucleoprotein Antibody Pair for Rapid COVID-19 Antigen Assays
High-Sensitivity SARS-CoV-2 Nucleoprotein Antibody Pair For Rapid COVID-19 Antigen Assays
In May 2020, the FDA announced the approval of the first antigen test to help in the rapid detection of the virus that causes COVID-19. Molecular tests are typically more sensitive and can diagnose an infection early in the process, but they must be carried out by trained technicians in a laboratory and they take time to report a result. Rapid antigen tests can provide a result in minutes and can be produced at a lower cost in large scales. Antigen tests are very specific for the virus but not as sensitive as PCR. A positive result by an antigen test is highly accurate, but a negative result does not rule out an infection. The performance of a rapid antigen test is limited by the sensitivity of the antibodies used. Meridian’s new pair of monoclonal antibodies are highly sensitive for the detection of SARS-CoV-2 Nucleoprotein and they do not cross-react with seasonal coronavirus strains. They are ideal for developing reliable and sensitive rapid lateral flow antigen assays for the detection of active COVID-19 infections.
SARS-CoV-2 Nucleoprotein Antibody Pair
Detects omicron variant down to 1.31 x 10 2 PFU/mL and all other variants down to 1.88 x 10 2 PFU/mL in clinical samples Used in both FDA EUA and CE-marked kits Validated for lateral flow & ELISA >95% purity (Protein G) Does not cross-react with other coronaviruses, except SARS (2003)
Cat# 9548
MAb to SARS-CoV-2 NP
Cat# 9547
MAb to SARS-CoV-2 NP
www.MeridianLifeScience.com
ELISA Performance, COVID-19 MAbs 9548 & 9547
Test Protocol
Add 100μl Sample dilution, incubate 37°C x 60 min STEP 1
STEP 2
STEP 3
STEP 4
STEP 5
Wash (3 cycles)
Add 100μl Conjugation dilution, incubate 37°C x 30 min
Wash (3 cycles)
Add 100μl TMB, incubate RT x 10 min
ELISA #1: Conjugate: COVID-19 MAb 9548 – HRP conjugate | Coating: COVID-19 MAb 9547 - 2μg/mL | Antigen: Rec. SARS-CoV-2 NP
Dilution
1/1,000
1/2,000
1/4,000
1/8,000
1/16,000
1/32,000
Blank
0.017 0.020 0.017 0.018 0.014 0.015 0.010 0.010 0.012 0.009 0.008 0.010 NaN NaN NaN NaN 3.711 3.749 2.680 2.715 1.758 1.812 1.046 1.194 3.881 NaN 3.482 3.586 3.033 3.077 2.249 2.259 1.397 1.414 0.869 0.920 3.733 3.387 2.879 2.933 2.409 2.488 1.776 1.782 0.896 1.055 0.569 0.711 2.970 2.692 2.290 2.334 1.865 1.956 1.333 1.330 0.814 0.806 0.447 0.481 2.006 1.887 1.514 1.585 1.204 1.201 0.820 0.846 0.513 0.533 0.282 0.293 0.254 0.241 0.143 0.192 0.138 0.135 0.080 0.087 0.050 0.054 0.029 0.028 0.029 0.024 0.016 0.016 0.014 0.015 0.017 0.017 0.008 0.013 0.008 0.011
10μg/mL 1μg/mL 500ng/mL 250ng/mL 100ng/mL 10ng/mL
1ng/mL
ELISA #2: Conjugate: COVID-19 MAb 9548 – HRP conjugate | Coating: COVID-19 MAb 9547 – 8μg/mL | Antigen: Rec. SARS-CoV-2 NP
Dilution
1/1,000
1/2,000
1/4,000
1/8,000
1/16,000
1/32,000
Blank
0.045 0.051 0.040 0.043 0.032 0.033 0.022 0.021 0.016 0.015 0.012 0.012 NaN NaN NaN NaN NaN NaN NaN NaN 2.886 2.883 1.717 1.814 NaN NaN NaN NaN NaN NaN 3.833 3.833 2.719 2.705 1.641 1.704 NaN NaN NaN NaN NaN NaN 3.502 3.439 2.280 2.347 1.386 1.429 NaN NaN NaN NaN 3.715 3.567 2.817 2.836 1.816 1.726 1.004 1.075 3.571 3.433 2.915 2.957 2.373 2.281 1.662 1.616 1.016 1.008 0.517 0.561 0.475 0.445 0.347 0.349 0.240 0.232 0.152 0.150 0.088 0.088 0.042 0.047 0.055 0.099 0.045 0.063 0.044 0.043 0.029 0.028 0.015 0.019 0.011 0.013
10ug/mL 1ug/mL 500ng/mL 250ng/mL 100ng/mL 10ng/mL
1ng/mL
Live Virus Detection Antibody pair 9547 and 9548 reacts with live virus. Antibodies were incorporated into a lateral flow assay and tested with approximately 2 x 10 3 TCID 50 mL inactivated COVID-19 virus (from Zeptomatrix, USA) and approx. 2 x 10 1 TCID 50 /mL live COVID-19 virus.
Lateral Flow Result
Control Line Test Line
Ordering information: USA 5171 Wilfong Road
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com
Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Connect with us:
ISO 13485 Certified
01/22
Page 1 Page 2Powered by FlippingBook