MAb Pair to SARS-CoV-2 NP

High-Sensitivity SARS-CoV-2 Nucleoprotein Antibody Pair For Rapid COVID-19 Antigen Assays

In May 2020, the FDA announced the approval of the first antigen test to help in the rapid detection of the virus that causes COVID-19. Molecular tests are typically more sensitive and can diagnose an infection early in the process, but they must be carried out by trained technicians in a laboratory and they take time to report a result. Rapid antigen tests can provide a result in minutes and can be produced at a lower cost in large scales. Antigen tests are very specific for the virus but not as sensitive as PCR. A positive result by an antigen test is highly accurate, but a negative result does not rule out an infection. The performance of a rapid antigen test is limited by the sensitivity of the antibodies used. Meridian’s new pair of monoclonal antibodies are highly sensitive for the detection of SARS-CoV-2 Nucleoprotein and they do not cross-react with seasonal coronavirus strains. They are ideal for developing reliable and sensitive rapid lateral flow antigen assays for the detection of active COVID-19 infections.

SARS-CoV-2 Nucleoprotein Antibody Pair

>95% purity (Protein G) Validated for Lateral Flow & ELISA Detects the new Alpha variant B.1.1.7 and Delta B.1.617.2 This antibody pair does not cross-react with other coronaviruses, except SARS (2003) Proven to react with live virus Tested in a commercial kit, achieves an LOD of 0.25 TCID 50 /mL

Cat# 9548

MAb to SARS-CoV-2 NP

Cat# 9547

MAb to SARS-CoV-2 NP

www.MeridianLifeScience.com

ELISA Performance, COVID-19 MAbs 9548 & 9547

Test Protocol

Add 100μl Sample dilution, incubate 37°C x 60 min STEP 1

STEP 2

STEP 3

STEP 4

STEP 5

Wash (3 cycles)

Add 100μl Conjugation dilution, incubate 37°C x 30 min

Wash (3 cycles)

Add 100μl TMB, incubate RT x 10 min

ELISA #1: Conjugate: COVID-19 MAb 9548 – HRP conjugate | Coating: COVID-19 MAb 9547 - 2μg/mL | Antigen: Rec. SARS-CoV-2 NP

Dilution

1/1,000

1/2,000

1/4,000

1/8,000

1/16,000

1/32,000

Blank

0.017 0.020 0.017 0.018 0.014 0.015 0.010 0.010 0.012 0.009 0.008 0.010 NaN NaN NaN NaN 3.711 3.749 2.680 2.715 1.758 1.812 1.046 1.194 3.881 NaN 3.482 3.586 3.033 3.077 2.249 2.259 1.397 1.414 0.869 0.920 3.733 3.387 2.879 2.933 2.409 2.488 1.776 1.782 0.896 1.055 0.569 0.711 2.970 2.692 2.290 2.334 1.865 1.956 1.333 1.330 0.814 0.806 0.447 0.481 2.006 1.887 1.514 1.585 1.204 1.201 0.820 0.846 0.513 0.533 0.282 0.293 0.254 0.241 0.143 0.192 0.138 0.135 0.080 0.087 0.050 0.054 0.029 0.028 0.029 0.024 0.016 0.016 0.014 0.015 0.017 0.017 0.008 0.013 0.008 0.011

10μg/mL 1μg/mL 500ng/mL 250ng/mL 100ng/mL 10ng/mL

1ng/mL

ELISA #2: Conjugate: COVID-19 MAb 9548 – HRP conjugate | Coating: COVID-19 MAb 9547 – 8μg/mL | Antigen: Rec. SARS-CoV-2 NP

Dilution

1/1,000

1/2,000

1/4,000

1/8,000

1/16,000

1/32,000

Blank

0.045 0.051 0.040 0.043 0.032 0.033 0.022 0.021 0.016 0.015 0.012 0.012 NaN NaN NaN NaN NaN NaN NaN NaN 2.886 2.883 1.717 1.814 NaN NaN NaN NaN NaN NaN 3.833 3.833 2.719 2.705 1.641 1.704 NaN NaN NaN NaN NaN NaN 3.502 3.439 2.280 2.347 1.386 1.429 NaN NaN NaN NaN 3.715 3.567 2.817 2.836 1.816 1.726 1.004 1.075 3.571 3.433 2.915 2.957 2.373 2.281 1.662 1.616 1.016 1.008 0.517 0.561 0.475 0.445 0.347 0.349 0.240 0.232 0.152 0.150 0.088 0.088 0.042 0.047 0.055 0.099 0.045 0.063 0.044 0.043 0.029 0.028 0.015 0.019 0.011 0.013

10ug/mL 1ug/mL 500ng/mL 250ng/mL 100ng/mL 10ng/mL

1ng/mL

Live Virus Detection Antibody pair 9547 and 9548 reacts with live virus. Antibodies were incorporated into a lateral flow assay and tested with approximately 2 x 10 3 TCID 50 mL inactivated COVID-19 virus (from Zeptomatrix, USA) and approx. 2 x 10 1 TCID 50 /mL live COVID-19 virus.

Lateral Flow Result

Control Line Test Line

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