ELISA Performance, COVID-19 MAbs 9548 & 9547
Test Protocol
Add 100μl Sample dilution, incubate 37°C x 60 min STEP 1
STEP 2
STEP 3
STEP 4
STEP 5
Wash (3 cycles)
Add 100μl Conjugation dilution, incubate 37°C x 30 min
Wash (3 cycles)
Add 100μl TMB, incubate RT x 10 min
ELISA #1: Conjugate: COVID-19 MAb 9548 – HRP conjugate | Coating: COVID-19 MAb 9547 - 2μg/mL | Antigen: Rec. SARS-CoV-2 NP
Dilution
1/1,000
1/2,000
1/4,000
1/8,000
1/16,000
1/32,000
Blank
0.017 0.020 0.017 0.018 0.014 0.015 0.010 0.010 0.012 0.009 0.008 0.010 NaN NaN NaN NaN 3.711 3.749 2.680 2.715 1.758 1.812 1.046 1.194 3.881 NaN 3.482 3.586 3.033 3.077 2.249 2.259 1.397 1.414 0.869 0.920 3.733 3.387 2.879 2.933 2.409 2.488 1.776 1.782 0.896 1.055 0.569 0.711 2.970 2.692 2.290 2.334 1.865 1.956 1.333 1.330 0.814 0.806 0.447 0.481 2.006 1.887 1.514 1.585 1.204 1.201 0.820 0.846 0.513 0.533 0.282 0.293 0.254 0.241 0.143 0.192 0.138 0.135 0.080 0.087 0.050 0.054 0.029 0.028 0.029 0.024 0.016 0.016 0.014 0.015 0.017 0.017 0.008 0.013 0.008 0.011
10μg/mL 1μg/mL 500ng/mL 250ng/mL 100ng/mL 10ng/mL
1ng/mL
ELISA #2: Conjugate: COVID-19 MAb 9548 – HRP conjugate | Coating: COVID-19 MAb 9547 – 8μg/mL | Antigen: Rec. SARS-CoV-2 NP
Dilution
1/1,000
1/2,000
1/4,000
1/8,000
1/16,000
1/32,000
Blank
0.045 0.051 0.040 0.043 0.032 0.033 0.022 0.021 0.016 0.015 0.012 0.012 NaN NaN NaN NaN NaN NaN NaN NaN 2.886 2.883 1.717 1.814 NaN NaN NaN NaN NaN NaN 3.833 3.833 2.719 2.705 1.641 1.704 NaN NaN NaN NaN NaN NaN 3.502 3.439 2.280 2.347 1.386 1.429 NaN NaN NaN NaN 3.715 3.567 2.817 2.836 1.816 1.726 1.004 1.075 3.571 3.433 2.915 2.957 2.373 2.281 1.662 1.616 1.016 1.008 0.517 0.561 0.475 0.445 0.347 0.349 0.240 0.232 0.152 0.150 0.088 0.088 0.042 0.047 0.055 0.099 0.045 0.063 0.044 0.043 0.029 0.028 0.015 0.019 0.011 0.013
10ug/mL 1ug/mL 500ng/mL 250ng/mL 100ng/mL 10ng/mL
1ng/mL
Live Virus Detection Antibody pair 9547 and 9548 reacts with live virus. Antibodies were incorporated into a lateral flow assay and tested with approximately 2 x 10 3 TCID 50 mL inactivated COVID-19 virus (from Zeptomatrix, USA) and approx. 2 x 10 1 TCID 50 /mL live COVID-19 virus.
Lateral Flow Result
Control Line Test Line
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